Zinc-Boron-PLGA biocomposite material: preparation, structural characterization, and in vitro assessment.

IF 1.2 4区 医学 Q4 DEVELOPMENTAL BIOLOGY
Maria Viorica Ciocîlteu, Ion Romulus Scorei, Gabriela Rău, Claudiu Nicolicescu, Andrei Biţă, Vladimir Lucian Ene, Andreea Simionescu, Adina Turcu-Ştiolică, Venera Cristina Dinescu, Johny Neamţu, Laurenţiu Mogoantă, George Dan Mogoşanu
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引用次数: 0

Abstract

Nowadays, the state-of-the-art discoveries in the field of delivery systems for therapeutic purposes have redefined the importance of biocompatible and biodegradable poly(lactic-co-glycolic acid (PLGA) nanocomposites. The study aimed to obtain a biocomposite material, with improved properties of its constituents [zinc-boron (Zn-B) complex and PLGA], by a simple, cost-effective method. The water∕oil∕water double emulsion technique allowed the adjustment of the synthesis parameters, to maximize the degree of Zn-B complex encapsulation. The morphological aspects of the samples were established by scanning electron microscopy (SEM). Particle size distribution was determined by dynamic light scattering (DLS). Morphology was typical for PLGA, spherical one. Depending on the synthesis conditions, the obtained particles have diameters between 10-450 nm. Zeta potential (ZP) showed that the particles have electronegative surface charge, offering a favorable perspective on aggregation, flocculation, and dispersion phenomena. It was observed, applying the design of experiments, that the particles size increased with increasing amounts of PLGA and polyvinyl alcohol (PVA), while ZP increased with higher PLGA and smaller PVA amounts in the formulation. The encapsulation efficiency was determined by ultra-high performance liquid chromatography∕mass spectrometry (UHPLC∕MS). The in vitro assessment was performed using Vero CCL-81 epithelial cell line and the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test. Zn-B-PLGA biocomposite has promising characteristics and can be used for future biomedical applications.

锌-硼-PLGA 生物复合材料:制备、结构表征和体外评估。
如今,用于治疗目的的给药系统领域的最新发现重新定义了生物相容性和可生物降解的聚乳酸-聚乙二醇酸(PLGA)纳米复合材料的重要性。这项研究旨在通过一种简单、经济有效的方法,获得一种生物复合材料,并改善其成分[锌-硼(Zn-B)复合物和聚乳酸乙二酸酯(PLGA)]的性能。水∕油∕水双乳液技术允许调整合成参数,以最大限度地提高锌-硼复合物的封装程度。样品的形态由扫描电子显微镜(SEM)确定。通过动态光散射(DLS)测定了粒度分布。形态是典型的 PLGA 球形。根据合成条件的不同,获得的颗粒直径在 10-450 nm 之间。Zeta 电位(ZP)显示,颗粒表面带有负电荷,这为聚集、絮凝和分散现象提供了有利的视角。应用实验设计法观察到,颗粒大小随 PLGA 和聚乙烯醇(PVA)用量的增加而增大,而 ZP 则随配方中 PLGA 用量的增加和 PVA 用量的减少而增大。封装效率是通过超高效液相色谱∕质谱法(UHPLC∕MS)测定的。体外评估采用 Vero CCL-81 上皮细胞系和 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基溴化四氮唑(MTT)试验。Zn-B-PLGA 生物复合材料具有良好的特性,可用于未来的生物医学应用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
1.70
自引率
20.00%
发文量
221
审稿时长
3-8 weeks
期刊介绍: Romanian Journal of Morphology and Embryology (Rom J Morphol Embryol) publishes studies on all aspects of normal morphology and human comparative and experimental pathology. The Journal accepts only researches that utilize modern investigation methods (studies of anatomy, pathology, cytopathology, immunohistochemistry, histochemistry, immunology, morphometry, molecular and cellular biology, electronic microscopy, etc.).
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