The buccal micronucleus cytome assay: New horizons for its implementation in human studies

IF 2.3 4区医学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Mutation research. Genetic toxicology and environmental mutagenesis Pub Date : 2024-01-06 DOI:10.1016/j.mrgentox.2023.503724

Michael Fenech , Siegfried Knasmueller , Armen Nersesyan , Claudia Bolognesi , Georg Wultsch , Christian Schunck , Emanuela Volpi , Stefano Bonassi

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Abstract

In this report we provide a summary of the presentations and discussion of the latest knowledge regarding the buccal micronucleus (MN) cytome assay. This information was presented at the HUMN workshop held in Malaga, Spain, in connection with the 2023 European, Environmental Mutagenesis and Genomics conference. The presentations covered the most salient topics relevant to the buccal MN cytome assay including (i) the biology of the buccal mucosa, (ii) its application in human studies relating to DNA damage caused by environmental exposure to genotoxins, (iii) the association of buccal MN with cancer and a wide range of reproductive, metabolic, immunological, neurodegenerative and other age-related diseases, (iv) the impact of nutrition and lifestyle on buccal MN cytome assay biomarkers; (v) its potential for application to studies of DNA damage in children and obesity, and (vi) the growing prospects of enhancing the clinical utility by automated scoring of the buccal MN cytome assay biomarkers by image recognition software developed using artificial intelligence. The most important knowledge gap is the need of prospective studies to test whether the buccal MN cytome assay biomarkers predict health and disease.

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口腔微核试验--人类研究的新视野

在本报告中，我们总结了有关口腔微核（MN）细胞组检测的最新知识。这些信息是在西班牙马拉加举行的与 2023 年欧洲、环境诱变和基因组学会议相关的 HUMN 研讨会上提供的。演讲内容涵盖了与口腔 MN 细胞组测定相关的最突出主题，包括：(i) 口腔黏膜生物学，(ii) 其在与环境暴露于遗传毒素导致的 DNA 损伤相关的人类研究中的应用，(iii) 口腔 MN 与癌症以及各种生殖、代谢、免疫、神经退行性疾病和其他老年相关疾病的关联，(iv) 营养和生活方式对口腔 MN 细胞组测定生物标志物的影响；(vi) 利用人工智能开发的图像识别软件对口腔 MN 细胞组检测生物标志物进行自动评分，从而提高临床实用性的前景日益看好。最重要的知识空白是需要进行前瞻性研究，以检验口腔 MN 细胞组测定生物标志物是否能预测健康和疾病。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Mutation research. Genetic toxicology and environmental mutagenesis 生物-毒理学

CiteScore

3.80

自引率

5.30%

发文量

审稿时长

105 days

期刊介绍： Mutation Research - Genetic Toxicology and Environmental Mutagenesis (MRGTEM) publishes papers advancing knowledge in the field of genetic toxicology. Papers are welcomed in the following areas: New developments in genotoxicity testing of chemical agents (e.g. improvements in methodology of assay systems and interpretation of results). Alternatives to and refinement of the use of animals in genotoxicity testing. Nano-genotoxicology, the study of genotoxicity hazards and risks related to novel man-made nanomaterials. Studies of epigenetic changes in relation to genotoxic effects. The use of structure-activity relationships in predicting genotoxic effects. The isolation and chemical characterization of novel environmental mutagens. The measurement of genotoxic effects in human populations, when accompanied by quantitative measurements of environmental or occupational exposures. The application of novel technologies for assessing the hazard and risks associated with genotoxic substances (e.g. OMICS or other high-throughput approaches to genotoxicity testing). MRGTEM is now accepting submissions for a new section of the journal: Current Topics in Genotoxicity Testing, that will be dedicated to the discussion of current issues relating to design, interpretation and strategic use of genotoxicity tests. This section is envisaged to include discussions relating to the development of new international testing guidelines, but also to wider topics in the field. The evaluation of contrasting or opposing viewpoints is welcomed as long as the presentation is in accordance with the journal''s aims, scope, and policies.