FLAVONOIDS OF THE FAR EASTERN SPECIES OF THE GENUS BUPLEURUM L.

chemistry of plant raw material Pub Date : 2023-06-26 DOI:10.14258/jcprm.20230211645

A. V. Myagchilov, P. Gorovoy, L. Sokolova

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Abstract

Compounds of the flavonoid class were isolated from six species of plants of the genus Bupleurum L. growing in the Russian Far East (Primorsky Krai, Magadan Region) by liquid extraction. Five compounds were identified by reversed-phase liquid chromatography: avicularin, rutin, narcissin, quercetin, isorhamnetin. Avicularin has been identified in the plants B. atargense, B. longiradiatum for the first time. The structure of compounds (avicularin, rutin, narcissin) isolated by preparative column chromatography on silica gel in the gradient elution mode with a mixture of solvents (carbon tetrachloride and ethyl alcohol) was proved by NMR-1H, 13C, 1H,13C-HMBC spectroscopy and mass spectrometry with ESI. The sum of flavonoids in the aerial part of six species of the genus Bupleurum L. was determined by differential spectrophotometry, the mass fraction of which varies from 0.58 to 2.92%. The highest content of flavonoids was found in B. euphoiroides – 2.92±0.58%, and the least – in B. longiradiatum (0.58±0.12%). Comparative analysis of individual flavonoids in species of the genus Bupleurum L. revealed the specificity of their accumulation. The main flavonoid in the aerial part of B. atargense, B. euphoiroides is quercetin – 1.14±0.23%, 0.92±0.18%, and in B. triradiatum, B. komarovianum, B. scorzonerifolium, B. longiradiatum – rutin (0.47±0.09%, 0.18±0.04%, 0.32±0.08%, 0.13±0.03% accordingly).

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柴胡属远东物种的黄酮类化合物

通过液体萃取法，从生长在俄罗斯远东地区（滨海边疆区、马加丹州）的六种柴胡属植物中分离出了黄酮类化合物。通过反相液相色谱法鉴定出五种化合物：水蛭素、芦丁、水仙素、槲皮素、异鼠李素。首次在 B. atargense 和 B. longiradiatum 两种植物中鉴定出了阿维菌素。通过 NMR-1H、13C、1H、13C-HMBC 光谱和 ESI 质谱分析，证明了在硅胶上使用混合溶剂（四氯化碳和乙醇）进行梯度洗脱的制备柱色谱法分离出的化合物（阿魏苷、芦丁、水仙苷）的结构。用微分分光光度法测定了六种羽扇豆属植物气生部分中黄酮类化合物的总和，其质量分数从 0.58% 到 2.92% 不等。在 B. euphoiroides 中发现的黄酮含量最高 - 2.92±0.58%，在 B. longiradiatum 中发现的黄酮含量最低 - 0.58±0.12%。Bupleurum L.属物种中单个黄酮类化合物的比较分析表明了其积累的特异性。在 B. atargense、B. euphoiroides 的气生部分，主要黄酮类化合物是槲皮素 - 1.14±0.23%、0.92±0.18%，而在 B. triradiatum、B. komarovianum、B. scorzonerifolium、B. longiradiatum 中则是芦丁（分别为 0.47±0.09%、0.18±0.04%、0.32±0.08%、0.13±0.03%）。

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chemistry of plant raw material

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