EVALUATION OF IN-VITRO ANTI-INFLAMMATORY AND ANTIBACTERIAL POTENTIAL OF METHANOLIC LEAF EXTRACTS OF ELYTRANTHE PARASITICA (L.) DANSER (LORANTHACEAE) – A HEMIPARASITIC ANGIOSPERM

Abstract

Elytranthe parasitica (L.) Danser is a hemiparasitic angiosperm shrub commonly called Macrosolen parasiticus and a member of the Loranthaceae family. The members of Loranthaceae are utilized globally in conventional medicine to cure a variety of ailments. The current study aimed to assess the qualitative screening of E. parasitica for its secondary metabolites and also in-vitro evaluation of anti-inflammatory and antibacterial potential. The fresh leaf samples were collected and extracted with the help of a soxhlet extractor. The in-vitro anti-inflammatory potential was determined by protein denaturation and HRBC membrane stabilization assays, and evaluation of antibacterial properties by agar well diffusion assay. The results of the qualitative evaluation of phytoconstituents revealed a presence of wide range of secondary metabolites. The methanolic leaf extracts of Elytranthe parasitica exhibited significant protein denaturation activity with 82.46 ± 1.37 % of inhibition of protein denaturation at 250 μg mL-1 concentration and showed significant membrane stabilization activity with 65.57 ± 2.60 % of membrane stabilization at 500 μg mL-1 concentration. It also shows considerable antibacterial potential with the maximum inhibition zone against Xanthomonas campestris (18.16 ± 0.44 mm) followed by Escherichia coli (15.33 ± 0.88 mm), Staphylococcus aureus (11.16 ± 0.44 mm), and Salmonella typhi (10.66 ± 0.66 mm) at higher concentrations. To conclude, it was found that E. parasitica (L.) Danser has a variety of secondary metabolites that have potent antiinflammatory and antibacterial properties based on the experiments carried out.