Sanative Effect of Newly Developed Herbal Formulation SS-IM-21 Upon Ethanol Induced Hepatic Oxidative Stress Against Mice

Abstract

Background and Objective: Oxidative stress represents an imbalance between oxidant and antioxidant agents. Reactive oxygen species (ROS) derived from oxygen and nitrogen play a vital role in the propagation of liver injury by damaging cell membranes, micro and macromolecules. The objective of the study of this study was to find out the probable ameliorative effect of the newly developed herbal formulation (SS-IM-21) on serum and hepatic antioxidant enzymes activities in ethanol-induced oxidative stress in mice. Materials and Methods: Forty adult Swiss albino mice were randomly chosen and divided into four groups, Group-I as control, Group II ethanol treatment orally (50% v/v), Group III pre-treated with herbal formulation (SS-IM-21) with ethanol (50% v/v) and Group-IV only treated with herbal formulation (SS-IM-21) without ethanol daily. After the 6 weeks of the experimental period, the animals were euthanized and the livers were immediately removed and collected, kept frozen until analysis. Before the mice were killed blood samples were also collected to measure superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione-S transferase (GST) activities in sera. Results: Major antioxidant enzymes like superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione-S transferase (GST) activities in serum and liver significantly declined in the ethanol-treated mice to control. Treatment with herbal formulation (SS-IM-21) suppresses oxidative stress in comparison with the ethanol group. Conclusion: In the current study the results established that treatment with herbal formulation (SS-IM-21) might be a potent antioxidant that exerts beneficial effects on both superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and glutathione-S transferase (GST) activities in ethanol intoxicated mice and inhibit organ damage.