Histological Evaluation of Skin Wound Healing After Using the Human Adipose Tissue-derived Stem Cells in Male Diabetic Rats

Jundishapur Journal of Medical Sciences Pub Date : 2023-07-01 DOI:10.32598/jsmj.21.6.2593

M. Nilforoushzadeh, Sona Zare, Elmira Zaraee, Rahim Ahmadi

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Abstract

Background and Objectives The use of stem cells for skin wound healing is still facing serious challenges. This study aims to determine the effectiveness of human adipose tissue-derived stem cells (ADSCs) spray in histologically improving skin wounds in male diabetic rats. Subjects and Methods In this experimental study, mesenchymal stem cells were isolated from adipose tissue and cultured and characterized by evaluating the expression of specific CD markers. Microbial contamination in samples was assessed before treatment. Then, 18 male Wistar rats were divided into control and treatment groups. Diabetes was induced by a single injection of streptozotocin. Then, wounds with a diameter of 0.8 cm were created in the back of the rats. The ADSCs were sprayed on the wound area in the treatment group. Wound healing was assessed on days 7, 14, and 21 after treatment using visual observation and histopathological examination. The data were analyzed using ANOVA. Results Wound closure was accelerated on days 7, 14 and 21 in the treatment group compared to the control group. Histological examination showed that the skin thickness, angiogenesis, and epithelialization were higher in the treatment group on days 7, 14, and 21 compared to the control group. Conclusion The spray of ADSCs on the wound area can accelerate the healing process and can be used for skin wound repair.

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雄性糖尿病大鼠使用人脂肪组织来源干细胞后皮肤伤口愈合的组织学评估

背景与目的干细胞用于皮肤伤口愈合仍面临严峻挑战。本研究旨在确定人类脂肪组织衍生干细胞（ADSCs）喷雾剂对雄性糖尿病大鼠皮肤伤口组织学改善的有效性。实验对象与方法在这项实验研究中，从脂肪组织中分离出间充质干细胞，并通过评估特定CD标记物的表达对其进行培养和表征。处理前对样本中的微生物污染进行了评估。然后，将 18 只雄性 Wistar 大鼠分为对照组和治疗组。通过单次注射链脲佐菌素诱发糖尿病。然后，在大鼠背部制造直径为 0.8 厘米的伤口。在治疗组的伤口处喷洒 ADSCs。在治疗后的第 7、14 和 21 天，通过肉眼观察和组织病理学检查评估伤口愈合情况。数据采用方差分析。结果与对照组相比，治疗组在第 7、14 和 21 天伤口闭合速度加快。组织学检查显示，与对照组相比，治疗组在第 7、14 和 21 天的皮肤厚度、血管生成和上皮化程度更高。结论在伤口部位喷洒 ADSCs 可加速愈合过程，可用于皮肤伤口修复。

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Jundishapur Journal of Medical Sciences

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