COMPARATIVE ANTIMICROBIAL ACTIVITY OF SOME METABIOTICS SYNTHESIZED BY LACTIC ACID BACTERIA

THE NEW ARMENIAN MEDICAL JOURNAL Pub Date : 2023-07-09 DOI:10.56936/18290825-2023.17.f-98

F. Tkhruni, A. Israyelyan

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Abstract

Abstract This paper presents the comparative characteristics of the antimicrobial activity of selected lactic acid bacteria strains and antibiotics. The metabiotics of probiotic lactic acid bacteria inhibited the growth of pathogenic, conditionally pathogenic bacteria, different etiology antibiotic resistant bacteria such as Salmonella sp., E. coli, Proteus mirabilis Pasteurella spp., Clostridium sp., Streptococcus sp., Staphylococcus aureus, Shigella sp., Yersinia enterocolitica, Bacillus cereus with different efficiency depending on pathogens isolation sources. It was shown that bacteriocins of lactic acid bacteria in the same concentration did not affect growth of the commensal microbiota strains, belonging to different genera and species. Lactobacillus and Enterococcus genera showed high sensitivity to investigated antibiotics (about 70%). Among all studied LAB strains of Enterococcus genus, some strains were shown to synthesize polysaccharides. The antimicrobial activity of isolated polysaccharides from Enterococcus faecium К Э-14, Enterococcus faecium К Э-5, Enterococcus lactic acid bacteria. Sp. К Э-9, Enterococcus lactic acid bacteria sp. К Э-6 strains was investigated. It was found that only polysaccharides isolated from Enterococcus faecium К Э-14 and Enterococcus faecium К Э-5 strains show an antimicrobial effect. The Enterococcus faecium КЭ-5 (MDC 9662) lactic acid bacteria strain was selected which produce protein-like substances and disaccharide polymers with antimicrobial activity, consist of glucose and galactose. The growth suppression of different Kl. pneumonia and St. pneumonia strains causing pneumonia by antimicrobial preparations of lactic acid bacteria was shown. The highest antimicrobial activity (100%) was observed when the antimicrobial preparations obtained after cultivation of lactic acid bacteria strains of the Enterococcus genus. The activity depends on the source of isolation of pathogens from a patient. The selected strains can be recommended for the creation of probiotic preparations with targeted purposes.

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乳酸菌合成的一些代谢物的抗菌活性比较

摘要本文介绍了所选乳酸菌菌株与抗生素抗菌活性的比较特性。益生乳酸菌的代谢物对致病菌、条件致病菌、不同病原的抗生素耐药菌如沙门氏菌属、大肠杆菌属、变形杆菌属、巴斯德氏菌属、梭状芽孢杆菌属、链球菌属、金黄色葡萄球菌属、志贺氏菌属、小肠结肠耶尔森菌属、蜡样芽孢杆菌属等的生长有抑制作用，抑制效率因病原体分离来源而异。研究表明，相同浓度的乳酸菌细菌素不会影响属于不同菌属和菌种的共生微生物群菌株的生长。乳酸菌属和肠球菌属对所研究的抗生素表现出较高的敏感性（约 70%）。在所有研究的肠球菌属 LAB 菌株中，有些菌株能合成多糖。从粪肠球菌 К Э-14、粪肠球菌 К Э-5、乳酸肠球菌 Enterococcus lactic acid bacteria.Sp.КЭ-9，乳酸肠球菌sp.К Э-6菌株进行了研究。研究发现，只有从粪肠球菌К Э-14和粪肠球菌К Э-5菌株中分离出的多糖具有抗菌作用。筛选出的粪肠球菌КЭ-5（MDC 9662）乳酸菌株能产生蛋白样物质和具有抗菌活性的双糖聚合物，由葡萄糖和半乳糖组成。结果表明，乳酸菌抗菌制剂能抑制引起肺炎的不同克氏肺炎菌株和圣肺炎菌株的生长。在培养肠球菌属乳酸菌菌株后获得的抗菌制剂的抗菌活性最高（100%）。抗菌活性取决于从患者体内分离病原体的来源。所选菌株可推荐用于制作有针对性的益生菌制剂。

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THE NEW ARMENIAN MEDICAL JOURNAL

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