Association between dental caries prevalence and Streptococcus mutans among 13-year-old children.

Abstract

The prevalence of caries associated prevalence with Streptococcus mutans in saliva and pooled plaque was investigated among 13-year-old Chinese children. In conjunction with saliva sampling simplified greatly by adopting the oral rinse method, an initial threshold value of 10(4) colony forming units (cfu) per ml of rinse was established on the basis of the S. mutans counts from 27 caries-free individuals. The results of the present study showed that, of the total 58 children, 67.3% had S. mutans counts above the threshold value in their saliva, and they developed significantly more decayed surfaces (D) and decayed, missing, filled surfaces (DMFS) than did the children below this value. The association between caries activity and S. mutans counts either in saliva or in pooled plaque samples was even stronger when only decayed surfaces were taken into account. In addition, the detection frequency of S. mutans (81.8%) was higher in saliva than in the pooled plaque samples (43.2%). This may demonstrate that saliva is more sensitive than dental plaque in predicting caries activity. The most prevalent biotypes of the S. mutans strains observed in this study were c and d. The results of this study indicate a significant association of S. mutans levels with caries prevalence. In the estimation of salivary S. mutans levels, the rinse method offered an easy and rapid identification for children with high caries risk and proved to be very practicable for epidemiological study on a larger scale.