Türkiye'nin güneyindeki meralardan Azotobacter izolasyon protokolleri kullanılarak Azotobacter olmayan bakterilerin izolasyonu ve moleküler karakterizasyonu

Biological Diversity and Conservation Pub Date : 2023-09-18 DOI:10.46309/biodicon.2023.1273996

Ebru Çelen

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Abstract

Isolation and molecular characterization of non-Azotobacter bacteria using Azotobacter isolation protocols from pastures in the south of Turkey Ebru ÇELEN ORCID: 0000-0002-8452-5933 Department of Biology, Faculty of Arts and Science, Bolu Abant İzzet Baysal University, Gölköy, 14280 Bolu, Türkiye Abstract Soil is a biodiversity-rich ecosystem. Nitrogen-fixing Azotobacter bacteria, an important component of this ecosystem, are frequently isolated using various methods. The aim of the present study was to perform partial molecular characterization of non-Azotobacter isolates derived during two Azotobacter isolation protocols, as well as to determine which bacteria can be obtained using this method. A total of 800 isolates were acquired from soil samples collected from various pastures in Antalya province of Turkey, with 92 of them being molecularly characterized. These isolates were clustered through RFLP analysis of 16S rRNA gene and the DNA sequences of the isolates representing different groups were performed. According to these results, the bacteria belonging to various genera (Agrobacterium, Phyllobacterium, Variovorax, Acinetobacter, Pseudomonas, Agromyces, and Arthrobacter) were identified. The results show that similar bacteria could be obtained through two isolation protocols used in this study. However, more diverse bacteria were encountered on the Brown-medium than on soil-past. Keywords: Azotobacter, isolate, soil, 16S rDNA ----------  ----------

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利用氮细菌分离方案从土耳其南部牧场分离非氮细菌并确定其分子特征

土耳其南部牧场非氮细菌的分离与分子鉴定 Ebru ÇELEN ORCID: 0000-0002-8452-5933 Department of Biology, Faculty of Arts and Science, Bolu Abant İzzet Baysal University, Gölköy, 14280 Bolu, Türkiye Abstract Soil is a biodiversity-rich ecosystem.固氮氮芽胞杆菌是这一生态系统的重要组成部分，人们经常使用各种方法对其进行分离。本研究的目的是对在两种根瘤菌分离方案中分离出的非根瘤菌进行部分分子鉴定，并确定哪些细菌可通过这种方法获得。本研究从土耳其安塔利亚省各种牧场采集的土壤样本中共获得 800 个分离株，对其中 92 个进行了分子鉴定。通过 16S rRNA 基因的 RFLP 分析对这些分离物进行了聚类，并对代表不同组别的分离物进行了 DNA 序列分析。根据这些结果，确定了属于不同菌属（农杆菌属、植物杆菌属、变种杆菌属、不动杆菌属、假单胞菌属、农杆菌属和节杆菌属）的细菌。结果表明，通过本研究中使用的两种分离方案可以获得类似的细菌。不过，与土壤上层相比，在布朗中层上发现的细菌种类更多。关键词氮杆菌分离物土壤 16S rDNA ----------  ----------

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Biological Diversity and Conservation

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