ACHIEVEMENTS IN PHYSICAL CHEMISTRY IN THE FIELD OF MICROSCOPY AND VISUALIZATION OF NANOSYSTEMS

Volodymyr Ogenko
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Abstract

The review presents modern views and the history of the development of microscopic studies of nanosystems which heve been started 2014, after the Nobel Prize in Chemistry was awarded to Eric Betzig, William Mörner, and Stefan Gell "for the development of super-resolved fluorescence microscopy". Their work ushered in a new era of optical microscopy, enabling the precise examination of individual molecules and molecular clusters by using optical microscopes. By circumventing the diffraction limitations that had constrained traditional optical microscopes, scientists gained access to the nanoscale realm, investigating structures within the 1–100 nanometer range. Special attention is paid to the use of carbon quantum dots and plasmon resonance to enhance fluorescence when obtaining the effect of super-resolution images, which allow the use of optical microscopes in the estimation of the sizes of cluster and single molecules. This breakthrough in removing the diffraction li­mitation allowed scientists to use the working range of 1–100 nm and obtain 3D images of nanosystems and images of living cells. Particular attention is paid to the achievements and prospects of high-resolution fluorescent nanoscopy SRM, which is successfully deve­lo­ping and studying the nanoworld in the range of 1–100 nm at the level of scanning electron microscopy. In cell biology, nanomedicine, etc. are developing roadmaps for scientific breakthroughs in super-resolution visualization me­thods for "live" images. Prospects of Immuno-­SERS microscopy and medicine of indivi­dual diagnosis are considered Key Findings: This article highlights the achievements and future prospects of super-resolution fluorescence microscopy SRM. High-resolution fluorescence microscopy has proven instrumental in advancing our understanding of the living world within the 1–100 nanometer range, which is akin to the capabilities of scanning electron microscopy. Within the domains of cell biology and nanomedicine, roadmaps for scientific breakthroughs are emerging, fueled by super-re­so­lution imaging techniques, providing "live" insights into cellular processes. The horizons of Immuno-SERS Microscopy and Personalized Diagnostics Medicine are expanding, promising exciting prospects in the field of medical diagnostics.
物理化学在纳米系统显微镜和可视化领域的成就
在埃里克-贝齐格、威廉-莫尔纳和斯特凡-盖尔因 "超分辨荧光显微技术的发展 "获得诺贝尔化学奖之后,本综述介绍了纳米系统显微研究的现代观点和发展历史。他们的工作开创了光学显微镜的新时代,使人们能够使用光学显微镜精确检查单个分子和分子团簇。通过规避制约传统光学显微镜的衍射限制,科学家们得以进入纳米级领域,研究 1-100 纳米范围内的结构。在获得超分辨率图像效果时,特别注意使用碳量子点和等离子体共振来增强荧光,从而可以使用光学显微镜来估算团簇和单分子的大小。在消除衍射限制方面取得的这一突破,使科学家能够使用 1-100 纳米的工作范围,获得纳米系统的三维图像和活细胞图像。特别值得关注的是高分辨率荧光纳米镜 SRM 的成就和前景,它在扫描电子显微镜的水平上成功地开发和研究了 1-100 纳米范围内的纳米世界。在细胞生物学、纳米医学等领域,正在开发 "活 "图像超分辨率可视化方法的科学突破路线图。免疫-SERS显微镜和个体诊断医学的前景被视为主要发现: 本文重点介绍了超分辨荧光显微技术 SRM 的成就和未来前景。 事实证明,高分辨率荧光显微镜有助于推进我们对 1-100 纳米范围内生物世界的了解,这与扫描电子显微镜的功能类似。 在细胞生物学和纳米医学领域,在超分辨率成像技术的推动下,科学突破的路线图正在形成,为细胞过程提供了 "实时 "的洞察力。 免疫-SERS 显微镜和个性化诊断医学的视野正在不断扩大,为医学诊断领域带来了令人兴奋的前景。
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