SPECTROPHOTOMETRIC STUDY OF COMPLEXES OF SOME INTERCALATORS WITH DOUBLE-STRANDED AND SINGLE-STRANDED NUCLEIC ACIDS

A. Antonyan, Zvart O. Movsisyan, Anzhela H. Karapetyan, P. Vardevanyan
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Abstract

Interaction of ethidium bromide (EtBr), acridine orange (AO) and non-intercalator methylene blue (MB) with DNA as well as interaction of EtBr and MB with single-stranded synthetic polynucleotides poly(rA) and poly(rU) have been studied. It was revealed that in absorption spectra of EtBr and MB with DNA or single-stranded polynucleotides a real- or pseudo-isosbestic point appears. Particularly, in absorption spectra of the complexes of classical intercalator EtBr with DNA the real isosbestic point is formed, but in the case of non-classical intercalator MB a pseudo-isosbestic point is formed, while in the absorption spectra of their complexes with single-stranded polynucleotides a pseudo-isosbestic point is formed. It was found out that for another classical intercalator AO there is no a real or pseudo-isosbestic point.
一些中间体与双链和单链核酸复合物的分光光度法研究
研究了溴化乙锭(EtBr)、吖啶橙(AO)和非交联剂亚甲基蓝(MB)与 DNA 的相互作用,以及 EtBr 和 MB 与单链合成多核苷酸 poly(rA) 和 poly(rU) 的相互作用。研究发现,在 EtBr 和甲基溴与 DNA 或单链多核苷酸的吸收光谱中,会出现一个实性或假性异色点。特别是,在经典插入剂 EtBr 与 DNA 的复合物的吸收光谱中,形成了真等距点,但在非经典插入剂 MB 的情况下,形成了假等距点,而在它们与单链多核苷酸的复合物的吸收光谱中,形成了假等距点。研究发现,另一种经典插入剂 AO 不存在真实或假性异斯巴达点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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