Multi-Bioactive Potential of a Rye Protein Isolate Hydrolysate by Enzymatic Processes

Foods 2023 Pub Date : 2023-10-14 DOI:10.3390/foods2023-15037
Desiree Islas-Martínez, Yair Noé Ávila-Vargas, G. Rodríguez-Serrano, L. González-Olivares, J. G. Pérez-Flores, E. Contreras-López, Enrique J. Olloqui, E. Pérez-Escalante
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Abstract

to this work. Abstract: Foods based on plant proteins have increased in recent years. Thus, the work aimed to establish the potential of rye protein hydrolysates to release bioactive peptides to treat non-communicable diseases. Rye protein isolate was obtained by solubilization-precipitation method and hydrolyzed with alcalase and flavourzyme. Hydrolysates were assessed for antioxidant, antihypertensive, and anti-diabetic properties. The rye protein isolate showed a protein content of 51.06 ± 1.15%, and its hydrolysates showed antioxidant, antihypertensive, and anti-diabetic capacities of 34.77 ± 0.59, 86.15 ± 1.15, and 40.62 ± 2.61% for flavourzyme, while for alcalase were 78.58 ± 1.04, 86.76 ± 2.02, and 63.70 ± 1.27%. Hydrolysis with alcalase was the greatest, and the multifunctionality obtained was better than in similar studies.
酶法水解黑麦蛋白分离物的多种生物活性潜力
到这项工作。摘要:近年来,基于植物蛋白的食品越来越多。因此,本研究旨在确定黑麦蛋白水解物释放生物活性肽治疗非传染性疾病的潜力。黑麦蛋白分离物是通过溶解沉淀法获得的,并用炼糖酶和黄酶进行水解。对水解物的抗氧化、抗高血压和抗糖尿病特性进行了评估。黑麦蛋白分离物的蛋白质含量为 51.06 ± 1.15%,其水解物的抗氧化、抗高血压和抗糖尿病能力分别为 34.77 ± 0.59%、86.15 ± 1.15%和 40.62 ± 2.61%(黄酮酶),而脂肪酶的水解物的抗氧化、抗高血压和抗糖尿病能力分别为 78.58 ± 1.04%、86.76 ± 2.02%和 63.70 ± 1.27%。使用乙醛酶的水解效果最好,所获得的多功能性也优于类似的研究。
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