Evaluation of Baicalein Loaded Hydrogel for Management of Diabetic Wound Healing

International Journal of Pharmaceutical Sciences and Drug Research Pub Date : 2023-10-20 DOI:10.25004/ijpsdr.2023.150505

K. D. Baviskar, S. Lodhi

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Abstract

The Present study aimed to perform a detailed in-vivo study of prepared baicalein (BCA) loaded hydrogel for wound healing effect and effect was compared with the marketed formulation. Prepared hydrogels were characterized and optimized already in previous study. Baicalein-loaded hydrogel (GG-GC-HGs) was prepared by using glycol chitosan gellan gum polymers. Prepared hydrogels were evaluated for wound healing effect using diabetic wound model (Streptozotocin induced) in rats. The wound healing effect was observed by measurement of wound contraction and biochemical estimation (Hydroxyproline, protein content and antioxidant assay) in the wound tissue after treatment. Histological examination of wound tissue was observed. Results of study showed that percent wound contraction of baicalein loaded GG-GC-HGs treated animal group showed a significant (p < 0.05) reduction after 10th day of treatment and healed completely on 18th day. Hydroxyproline and protein content were increased significantly with treatment of baicalein-loaded GG-GC-HGs and results were comparable with reference group (Hydroheal Gel) of animals. Antioxidant status was restored after treatment with BCA loaded GG-GC-HGs. The histological observation of wound tissues supported these results. In conclusion, baicalein loaded hydrogel showed prominent wound healing effect through increasing epithelialization, fibroblast proliferation and reducing oxidative stress in diabetic condition.

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评估含黄芩素水凝胶在糖尿病伤口愈合管理中的应用

本研究旨在对所制备的黄芩素（BCA）负载水凝胶的伤口愈合效果进行详细的体内研究，并将其效果与市场上的配方进行比较。制备的水凝胶已在之前的研究中进行了表征和优化。使用乙二醇壳聚糖结冷胶聚合物制备了黄芩素负载水凝胶（GG-GC-HGs）。利用糖尿病大鼠伤口模型（链脲佐菌素诱导）对制备的水凝胶的伤口愈合效果进行了评估。伤口愈合效果是通过测量伤口收缩和处理后伤口组织的生化评估（羟脯氨酸、蛋白质含量和抗氧化剂检测）来观察的。还对伤口组织进行了组织学检查。研究结果表明，黄芩苷负载 GG-GC-HGs 治疗动物组的伤口收缩率在治疗第 10 天后显著降低（p < 0.05），并在第 18 天完全愈合。黄芩素负载 GG-GC-HGs 治疗后，羟脯氨酸和蛋白质含量明显增加，结果与参照组（水合凝胶）动物相当。使用含 BCA 的 GG-GC-HGs 治疗后，抗氧化状态得到恢复。对伤口组织的组织学观察也证实了这些结果。总之，负载黄芩素的水凝胶通过增加糖尿病患者的上皮化、成纤维细胞增殖和减少氧化应激，显示出显著的伤口愈合效果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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International Journal of Pharmaceutical Sciences and Drug Research

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