Preliminary Pharmacological Screening of Corchorus olitorius Extracts for Anti-inflammatory and Wound Healing Activity

International Journal of Pharmaceutical Sciences and Drug Research Pub Date : 2023-10-20 DOI:10.25004/ijpsdr.2023.150502

P. C. Pradhan, M. Kori

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Abstract

Aim of present study was to investigate the preliminary screening of aerial part of Corchorus olitorius extracts for anti-inflammatory and wound-healing activity on experimental animals. Different extracts with respective solvents i.e. petroleum ether, chloroform, ethyl acetate, ethanol and aqueous extracts of C. olitorius were obtained by successive solvent extraction. All extracts were screened for anti-inflammatory activity using Xylene-induced ear edema in mice and wound healing effect was studied by using incision wound model in mice. The weight of the ear lobes and biochemical measurements, nitric oxide (NO) levels and MPO in the tissue sample were used to detect the anti-inflammatory effect. The wound healing effect was observed by measurement of tensile strength and protein and hydroxyproline level assessment in the healed tissues. Observations of present study were confirmed that ethanol extract of C. olitorius (EECO) was showed significant (p<0.05) inhibition in the ear edema of mice. NO and MPO activity was decreased significantly in EECO-treated mice. Wound healing potential was observed by significant improvement in tensile strength as well as protein and hydroxyproline level of healed tissue of EECO-treated mice. In conclusion, ethanol extract of C. olitorius was found most effective for anti-inflammatory through reduction of NO and MPO activity as well as wound healing potential in experimental mice.

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欧石蒜提取物抗炎和伤口愈合活性的初步药理筛选

本研究的目的是初步筛选欧石蒜气生部分提取物对实验动物的抗炎和伤口愈合活性。通过连续溶剂萃取法获得了不同的提取物和相应的溶剂，即石油醚、氯仿、乙酸乙酯、乙醇和水提取物。利用二甲苯诱导的小鼠耳部水肿对所有提取物进行了抗炎活性筛选，并利用小鼠切口模型研究了其伤口愈合效果。耳叶重量和生化指标、一氧化氮（NO）水平和组织样本中的 MPO 被用来检测抗炎效果。通过测量愈合组织的拉伸强度、蛋白质和羟脯氨酸水平评估，观察伤口愈合效果。本研究的观察结果表明，蛇床子乙醇提取物（EECO）对小鼠耳部水肿有显著的抑制作用（p<0.05）。经 EECO 处理的小鼠体内 NO 和 MPO 活性明显降低。经 EECO 处理的小鼠愈合组织的抗张强度、蛋白质和羟脯氨酸水平均有明显改善，从而观察到伤口愈合潜力。总之，通过降低小鼠体内的 NO 和 MPO 活性以及伤口愈合潜能，奥利司他的乙醇提取物被认为是最有效的抗炎药物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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International Journal of Pharmaceutical Sciences and Drug Research

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