Neuroprotective Activity of Premna latifolia on Streptozotocin Induced Diabetic Neuropathy in Rats

Abstract

The leaves of plant Premna latifolia Lam are traditionally used for treating diabetes and related complications. Presently ethanolic extract of leaves of plant P. latifolia explored for the neuroprotective potential in rat model of streptozotocin induced diabetic neuropathy. The intra-peritoneal route administered streptozotocin 60 mg/kg body weight to induce experimental diabetes, then treatment were not given for two weeks to develop diabetic complications. After two weeks of induction, animals were treated with extract 100 and 200 mg/kg per day for the duration of five weeks. The neuroprotective effect was assessed using Eddy’s hot plate and tail immersion method. Also, measuring blood sugar and cytokines cause inflammations like interleukins, tumor necrosis factor and a neurotrophin in the sciatic nerve. Thiobarbituric acid reactive substances reduced glutathione and activity levels of catalase, superoxide dismutase, glutathione peroxidase and glutathione reductase measured in the sciatic tissue. Extract normalizes blood glucose in diabetic neuropathy rats brought on by streptozotocin. The diabetic neuropathy rats show significant reductions in the period when the tail retracts and the paws are licked or jumped. In these rats pro-inflammatory cytokines level in sciatic nerve was significantly high. In addition, the oxidative stress bio-markers level altered significantly in sciatic nerve tissue. Diabetic neuropathy-developing rats treated with extract for five weeks reduce the levels of nerve growth factor, oxidative stress indicators, and pro-inflammatory cytokines rise in the sciatic nerve tissue. Ethanolic extract of P. latifolia possesses neuroprotective action in streptozotocin-induced diabetic neuropathy.