The Effect of Some Different Blood Anticoagulants on Rabbits Plasma Biochemical Parameters under Different Temperatures

Cardiometry Pub Date : 2023-11-01 DOI:10.18137/cardiometry.2023.29.111115

E.N.J. Al-Obaidy, M.M. Ismail, M.Z. Muhi

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Abstract

The aim of present study was to compare the effect of Ca chelating anticoagulants (EDTA, citrate and oxalate) and antithrombin anticoagulant (heparin) on some rabbits plasma biochemical parameters at room temperature 25℃ and extreme temperatures -18℃and 50℃ for 1hr. Five male rabbits weighting between 800-1000gm, kept in the animal house /College of Veterinary Medicine/University of Diyala, under 12:12 dark light cycle with ad libtum food and water in individual cages (60cm,40cm) during November and December /2022. Four ml of blood samples were collected by cardiac puncture (23G needle) into four tubes contain anticoagulants: (EDTA, citrate, oxalate and heparin)one ml of blood for each tube; then the blood samples were kept at :room temperature 25℃, waterbath50℃ and refrigerator -18 ℃, for 1hr then put in centrifuge at 3000rpm for 15min. From total examined blood samples =24 for each Ca chelating anticoagulants (EDTA, citrate and oxalate) and antithrombin anticoagulant (heparin) after 1hr under in 25c℃ , -18℃ and 50℃. The both groups of anticoagulants (Ca chelating and antithrombin) showed no significant differences P>0.05 between them in level of biochemical parameters (glucose, total protein, cholesterol and urea) after 1 hr at 25C; on the other hand significant increase in plasma glucose, total protein and cholesterol beside significant decrease in plasma urea of Ca chelating anticoagulants after 1hr at -18C and 50C as compare with antithrombin anticoagulant. In conclusion; heparin was more effective than Ca chelating anticoagulant in preserve plasma biochemical parameters within normal values under extreme cool and hot condition.

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不同温度下不同血液抗凝剂对家兔血浆生化指标的影响

本研究旨在比较钙螯合抗凝剂（乙二胺四乙酸二乙酯、柠檬酸盐和草酸盐）和抗凝血酶抗凝剂（肝素）在室温 25℃和极端温度 -18℃ 和 50℃下 1 小时对兔子血浆生化指标的影响。5 只体重在 800-1000gm 之间的雄性兔子，于 2022 年 11 月和 12 月期间饲养在迪亚拉大学兽医学院的动物房中，12:12 暗光周期，在单个笼子（60 厘米，40 厘米）中自由进食和饮水。通过心脏穿刺（23G 针头）采集四毫升血液样本，分别装入四支含有抗凝剂（乙二胺四乙酸、柠檬酸盐、草酸盐和肝素）的试管中，每支试管一毫升血液；然后将血液样本分别在室温 25℃、水浴 50℃和冰箱 -18 ℃ 下保存 1 小时，再放入 3000rpm 离心机中离心 15 分钟。在 25℃、-18℃ 和 50℃条件下放置 1 小时后，对钙螯合抗凝剂（乙二胺四乙酸、柠檬酸盐和草酸盐）和抗凝血酶抗凝剂（肝素）各取 24 份血样。两组抗凝剂（钙螯合剂和抗凝血酶）在 25C 下 1 小时后的生化指标（葡萄糖、总蛋白、胆固醇和尿素）差异不显著（P>0.05）；另一方面，与抗凝血酶抗凝剂相比，在 -18C 和 50C 下 1 小时后，钙螯合剂抗凝剂的血浆葡萄糖、总蛋白和胆固醇显著增加，血浆尿素显著减少。总之，在极冷和极热条件下，肝素比钙螯合抗凝剂更能有效地将血浆生化指标保持在正常值范围内。

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来源期刊

Cardiometry MEDICAL LABORATORY TECHNOLOGY-

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0.00%

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审稿时长

6 weeks

期刊介绍： Cardiometry is an open access biannual electronic journal founded in 2012. It refers to medicine, particularly to cardiology, as well as oncocardiology and allied science of biophysics and medical equipment engineering. We publish mainly high quality original articles, reports, case reports, reviews and lectures in the field of the theory of cardiovascular system functioning, principles of cardiometry, its diagnostic methods, cardiovascular system therapy from the aspect of cardiometry, system and particular approaches to maintaining health, engineering peculiarities in cardiometry developing. The interdisciplinary areas of the journal are: hemodynamics, biophysics, biochemistry, metrology. The target audience of our Journal covers healthcare providers including cardiologists and general practitioners, bioengineers, biophysics, medical equipment, especially cardiology diagnostics device, developers, educators, nurses, healthcare decision-makers, people with cardiovascular diseases, cardiology and engineering universities and schools, state and private clinics. Cardiometry is aimed to provide a wide forum for exchange of information and public discussion on above scientific issues for the mentioned experts.