Enhanced Production of Microbial Lipid from R. opacus using Bagasse Hydrolysate

Abstract

One alternative to traditional fossil fuels is biodiesel. It has a negative impact on nutritional supply because it is mostly manufactured from vegetable oils. Due to the possibility that oleaginous microbe lipids (such as those produced by bacteria, fungi and yeasts) could serve as an alternative raw material for the synthesis of biodiesel, the biomass and lipid buildup of Rhodococcus opacus were examined in several environmental settings. The study's current objective is to use the Response surface methodology's central composite design (CCD) to discover the ideal values of specific variables. Rhodococcus opacus, an oleaginous bacterium, was subjected to a CCD design using independent variables such as inoculum ratio, nitrogen concentration and magnesium concentration. The response variable used for the analysis was lipid content. Regarding the experimental data's lipid content%, a polynomial with a second-order model yielded good results (R2 = 96, (P ≤ 0.01)). Rhodococcus opacus produced the best results in the trials with glucose and hydrolysate with biomass concentrations of 4.75 g/L and 4.55 g/L and lipid contents of 71.18% and 66.55% respectively at pH 7. Compared to the starting values prior to optimization, these demonstrate a boost in the lipid concentration and an enhancement in the lipid coefficient. This study further supports the viability of R. opacus as a variant for the generation of biofuel from readily available, low-cost biomass resources.