Population genetic structure of Kawakawa (Euthynnus affinis Cantor, 1849) in Malaysian waters based on COI gene

IF 0.7 Q4 BIOLOGY

Nusantara Bioscience Pub Date : 2023-11-10 DOI:10.13057/nusbiosci/n150213

Kh Binashikhbubkr, A. Setyawan, D. Naim

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Abstract

Abstract. Binashikhbubkr K, Setyawan AD, Naim DM. 2023. Population genetic structure of Kawakawa (Euthynnus affinis (Cantor, 1849)) in Malaysian waters based on COI gene. Nusantara Bioscience 15: 258-268. Kawakawa (Euthynnus affinis Cantor, 1849) is widely distributed in the subtropical and tropical waters of the Indo-Pacific region. Still, insufficient data about its stock, management, and protection in Malaysia and nearby waters raises concerns about overfishing and depletion. Therefore, to ensure effective and successful management of a species, it is imperative to conduct a molecular-based assessment of the stock structure. The present study investigated the population genetic structure of E. affinis in Malaysian waters using the mtDNA COI gene. Furthermore, the 632 bp segment of the COI region was sequenced in 372 individuals from 19 distinct populations in Malaysian waters. The results revealed that the genetic divergence varied from low to high. The average Haplotype diversity (Hd) and nucleotide diversity (?) were calculated to be 0.5401 and 0.0045, respectively. Examining haplotype distribution unveiled the presence of 22 unique haplotypes within the COI gene of E. affinis. The analysis of the Neighbor Joining (NJ) tree and the Minimum Spanning Network (MSN) revealed the formation of three distinct clades among E. affinis samples. Analysis of Molecular Variance (AMOVA) showed a significant genetic structure among the 19 populations of E. affinis [(FST = 0.5354 (P < 0.05)]. The neutrality test and mismatch distribution analysis indicated that the specimens underwent a period of population expansion. This study is a significant milestone, providing the first comprehensive documentation of the genetic structure of E. affinis in Malaysia.

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基于 COI 基因的马来西亚水域川鱼（Euthynnus affinis Cantor, 1849）种群遗传结构

摘要： Binashikhbubkr K、Setyawan AD、Naim DM.Binashikhbubkr K, Setyawan AD, Naim DM.2023.基于 COI 基因的马来西亚水域川鱼（Euthynnus affinis (Cantor, 1849)）种群遗传结构。Nusantara Bioscience 15: 258-268.川鱼（Euthynnus affinis Cantor，1849 年）广泛分布于印度洋-太平洋地区的亚热带和热带水域。然而，马来西亚及其附近水域有关其种群、管理和保护的数据不足，这引起了人们对过度捕捞和枯竭的担忧。因此，为了确保有效和成功地管理一个物种，必须对其种群结构进行分子评估。本研究利用 mtDNA COI 基因调查了马来西亚水域 E. affinis 的种群遗传结构。此外，还对马来西亚水域 19 个不同种群中 372 个个体的 632 bp COI 区段进行了测序。结果显示，遗传差异从低到高不等。计算得出的平均单倍型多样性（Hd）和核苷酸多样性（?）分别为 0.5401 和 0.0045。对单倍型分布的研究发现，E. affinis 的 COI 基因中存在 22 个独特的单倍型。通过分析邻接树（NJ）和最小跨度网络（MSN），发现在E. affinis样本中形成了三个不同的支系。分子方差分析（AMOVA）显示，E. affinis 的 19 个种群之间存在显著的遗传结构[（FST = 0.5354 (P < 0.05)]。中性检验和错配分布分析表明，标本经历了一个种群扩张时期。这项研究是一个重要的里程碑，首次全面记录了马来西亚 E. affinis 的遗传结构。

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来源期刊

Nusantara Bioscience BIOLOGY-

自引率

25.00%

发文量

审稿时长

6 weeks