Silver Nanoparticles Coated with Recombinant Human Epidermal Growth Factor: Synthesis, Characterization, Liberation and Anti-Escherichia coli Activity

Reactions Pub Date : 2023-11-15 DOI:10.3390/reactions4040041
Layla M. Gonzales Matushita, Luis Palomino, J. Rodriguez-Reyes
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Abstract

Epithelial tissue regeneration may be favored if the tissue receives both therapeutic agents such as recombinant human epidermal growth factor (rhEGF) and, simultaneously, antibacterial materials capable of reducing the risk of infections. Herein, we synthesized silver nanoparticles (AgNPs), which are well-known antibacterial materials, and impregnate them with rhEGF in order to study a bio-nanomaterial of potential interest for epithelial tissue regeneration. A suspension of Ag NPs is prepared by the chemical reduction method, employing sodium citrate as both a reducer and capping agent. The AgNPs suspension is mixed with a saline solution containing rhEGF, producing rhEGF-coated Ag NPs with rhEGF loadings between 0.1 and 0.4% w/w. ELISA assays of supernatants demonstrate that, in all studied cases, over 90% of the added rhEGF forms part of the coating, evidencing a high efficiency in impregnation. During the preparation of rhEGF-coated Ag NPs, no significant changes are observed on the nanoparticles, which are characterized by UV-Vis spectroscopy, transmission electron microscopy (TEM) and infrared spectroscopy. The liberation of rhEGF in vitro was followed for 72 h, finding that approximately 1% of rhEGF that is present is released. The rhEGF-coated AgNPs shows antibacterial activity against E. coli, although such activity is decreased with respect to that observed from naked AgNPs. Having confirmed the possibility of simultaneously liberating rhEGF and reducing the proliferation of bacteria, this work helps to support the use of rhEGF-loaded metallic nanoparticles for tissue regeneration.
涂有重组人表皮生长因子的银纳米粒子:银纳米粒子的合成、表征、释放和抗大肠杆菌活性
如果上皮组织既能接受重组人表皮生长因子(rhEGF)等治疗药物,同时又能接受能降低感染风险的抗菌材料,上皮组织的再生就会变得更加有利。在此,我们合成了银纳米粒子(AgNPs)这种著名的抗菌材料,并用 rhEGF 对其进行浸渍,以研究一种对上皮组织再生具有潜在意义的生物纳米材料。采用柠檬酸钠作为还原剂和封盖剂,通过化学还原法制备 AgNPs 悬浮液。将 AgNPs 悬浮液与含有 rhEGF 的生理盐水溶液混合,产生 rhEGF 包覆的 Ag NPs,rhEGF 含量在 0.1% 到 0.4% w/w 之间。上清液的 ELISA 检测表明,在所有研究案例中,90% 以上的添加 rhEGF 都形成了涂层的一部分,证明浸渍效率很高。在制备 rhEGF 包覆的 Ag NPs 的过程中,纳米粒子没有发生明显变化,紫外可见光谱、透射电子显微镜(TEM)和红外光谱对其进行了表征。对体外释放 rhEGF 的情况进行了 72 小时的跟踪研究,发现大约有 1%的 rhEGF 被释放。包覆 rhEGF 的 AgNPs 对大肠杆菌具有抗菌活性,但与裸 AgNPs 相比,这种活性有所降低。这项工作证实了同时释放 rhEGF 和减少细菌增殖的可能性,有助于支持将负载 rhEGF 的金属纳米粒子用于组织再生。
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CiteScore
2.70
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