Changes of erythrocytes, hemoglobin and erythrocyte indices in cats with enterocolitis of unknown etiology

L. Karpenko, A. Bakhta, P. Pogodaeva, P. Bokhan
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Abstract

Purpose: Study red blood parameters in a group of cats with enterocolitis of unknown etiology using standard hematological research methods.Materials and methods. During the study, standard methods of hematological analysis were used: erythrocyte counting was performed in a Goryaev chamber using whole blood diluted with isotonic sodium chloride solution 200 times, counting was carried out using a light-optical microscope Miromed-2 var. 3-20 inf. lens x8, eyepiece x10; the amount of hemoglobin was determined by the colorimetric method, diluting whole blood 200 times with a solution of 0,04 % ammonia on a Microlab-540 device; hematocrit value was determined by centrifugation using a Vilitek DSC-100MH-2 hematocrit centrifuge; the reaction to the erythrocyte sedimentation rate was performed in Panchenkov pipettes; erythrocyte indices and blood color index were calculated using generally accepted formulas.Results. Thanks to the data obtained, we concluded that disruption of enzymatic processes, deterioration of absorption in the intestine and imbalance of symbiont microflora caused by enterocolitis can lead to the development of anemic conditions, manifested by changes in red blood parameters.
病因不明的猫小肠结肠炎患者红细胞、血红蛋白和红细胞指数的变化
目的:使用标准血液学研究方法研究一组病因不明的肠炎猫的红细胞参数。在研究过程中,使用了标准的血液分析方法:红细胞计数在高里耶夫室中进行,使用等渗氯化钠溶液稀释 200 倍的全血;计数使用 Miromed-2 var.用 Microlab-540 设备将全血用 0.04 % 氨溶液稀释 200 倍,用比色法测定血红蛋白量;用 Vilitek DSC-100MH-2 血细胞比容离心机离心测定血细胞比容值;用 Panchenkov 移液管进行红细胞沉降率反应;用公认公式计算红细胞指数和血色指数。根据所获得的数据,我们得出结论:肠炎引起的酶过程破坏、肠道吸收恶化和共生微生物菌群失衡会导致贫血症的发生,表现为红细胞参数的变化。
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