BİR İNFLUENZA VİRÜSÜ NÖRAMİNİDAZ İNHİBİTÖRÜ OLAN PERAMIVİR’İN SAPTANMASI İÇİN MOLEKÜLER BASKI POLİMER İLE ELEKTROKİMYASAL SENSÖRÜN GELİŞTİRİLMESİ

Abstract

Objective: Influenza viruses are the most common cause of influenza. Antiviral drugs prevent the spread of the virus through the infected cells. Peramivir is one of the antiviral drugs that is an inhibitor of influenza virus neuraminidase. In our study, we aimed to develop a MIP-based electrochemical sensor to determine Peramivir. This study is the first to create an electrochemical sensor for Peramivir. MIP(PERA)/GCE was fabricated with the electropolymerization of 4-aminophenol (4-AP) and ortophenilendiamine (o-PD) in the presence of Peramivir. The developed MIP(PERA)/GCE was applied to the commercial serum sample for analysis of Peramivir. Material and Method: PERA is supplied by Tobio Novelpharma pharmaceutical company (İstanbul, Türkiye). Potassium ferricyanide ([K3Fe(CN)6]), potassium ferrocyanide (K4[Fe(CN)6].3H2O), and potassium chloride (KCl), 4-aminophenol (4-AP) and ortophenilendiamine (o-PD), commercial human serum sample, dopamine, ascorbic acid, uric acid, paracetamol, KNO3, Na2SO4 ve MgCl2 were obtained from Sigma-Aldrich (St. Louis, Missouri, USA). Methanol, acetic acid, oxalic acid, hydrochloric acid, acetonitrile, sodium hydroxide, and ethanol were purchased by Merck. The redox process was investigated using cyclic voltammetry (CV) and differential pulse voltammetry (DPV) by the Dropsens μStat 400 Bipotantiostat/ Galvanostat (Metrohm, Herisau, Switzerland). Three-electrode systems consisted of a glassy carbon working electrode (GCE, 3mm2, BASi, USA), a saturated Ag/AgCl reference electrode, and a Pt wire. Result and Discussion: The sensor exhibits a linear range of 1-10 pM with a detection limit of 0.158 pM. The MIP(PERA)/GCE sensor could detect PERA from commercial serum samples with a high recovery of 101.81%.