Lipid peroxidation and the antioxidant system of the lungs during hyperbaric oxidation and its aftereffect: prospective study

Abstract

OBJECTIVE. To study the state of lipid peroxidation (LP) and the antioxidant system in the lungs of a healthy organism with single and repeated use of HBO in the therapeutic regime and the dynamics of the detected changes in the posthyperoxic period. MATERIALS AND METHODS. The experiments were conducted on a 100 mongrel white rats (males) weighing 180-220 g. Hyperbaric oxygenation (HBO) was performed in a therapeutic “soft” mode (2 ata, 50 min), 1 session per day. The studies were carried out immediately after the first and fifth sessions of HBO, as well as on the 5th day after a single application of HBO and on the 5th knock after the end of the five-day course of HBO. The content of extraerythrocytic hemoglobin (EEG), malondialdehyde (MDA), urea, uric acid (UC), activity of superoxide dismutase (SOD) and catalase were studied in the lung tissue. RESULTS. A five-day course of HBO does not lead to the accumulation of EEG in the lung tissue and causes an increase in the concentration of MDA in it only after the 5th session of HBO, whereas on the 5th day of the posthyperoxic period (PHP) it is within the normal range. Adapting to the selected HBO regimen, the lung tissue responds by increasing the activity of SOD and catalase, which normalize with an increase in the number of sessions to five. Unlike SOD, the increase in catalase activity on the 5th day of PHP depends on the number of HBO sessions. If the HBO-determined accumulation of urea by the lung tissue persists on the 5th day of the post-peroxic period, then the accumulation of UC is only with hyperoxic exposure. DISCUSSION. The changes we have identified are a manifestation of the natural reaction of the LP and the antioxidant system of the lungs of a healthy organism to an over-saturation with oxygen carried out in a therapeutic “soft” mode of 2 ata, 50 min. CONCLUSION. A five-day course of HBO in a therapeutic “soft” mode (2 ata, 50 min, 1 session per day) does not cause uncontrolled activation of POL and depletion of the antioxidant system in the lung tissue.