Effects of drought on enzyme activities and hotspot distribution along plant roots

IF 2.4 Q2 GEOSCIENCES, MULTIDISCIPLINARY
Luu Anh The, Mai Van Dinh, Do Trung Quang, Ali Feizi, Hoang Duyen Thi Thu
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Abstract

The frequency and severity of drought are projected to increase due to climate change, and Southeast Asia is no exception. Water scarcity hampers all biochemical processes in soil and induces stunted plant growth. While the rhizosphere harbors the most dynamic biochemical processes in the biosphere, the interaction mechanisms between residing microbes and plant roots under drought are poorly understood. In this research, soybean was planted in soil collected from the Red River Delta of Vietnam to test two hypotheses: (i) drought reduces rhizosphere enzyme activities and hampers the extent of the high enzyme activity along single root (from the root tips), and (ii) the turnover time of substrate by enzymes increases with decreasing soil moisture. The research aimed to characterize distributions of β-glucosidase and acid phosphatase enzymes in a distance from root tips. In addition, enzyme activities and plant root and shoot characteristics (length and weight) were investigated. The results demonstrated that shoot length was more impacted by drought than root length with the reduction of 25% for the former and 5% for the later. Meanwhile, the reduction in shoot weight was 61%, and root weight was 90% as the plant experienced drought conditions. The extent of a hotspot for enzymes along a single plant root, measured from the root tips, also decreased in response to drought. Furthermore, drought reduced both rhizosphere enzyme activities, resulting in a slower turnover time of β-D-glucopyranoside (MUF-G) and 4-methylumbelliferyl-phosphate(MUF-P) substrates. The research has shed light on the adverse impacts of drought on root-microbe interactions, which ultimately lead to poor crop growth.
干旱对植物根部酶活性和热点分布的影响
由于气候变化,干旱的频率和严重程度预计都会增加,东南亚也不例外。缺水阻碍了土壤中的所有生化过程,导致植物生长迟缓。根圈是生物圈中最活跃的生化过程,但人们对干旱条件下根圈微生物与植物根系之间的相互作用机制却知之甚少。本研究将大豆种植在从越南红河三角洲采集的土壤中,以验证两个假设:(i) 干旱会降低根瘤菌酶的活性,并阻碍单根(从根尖开始)高酶活性的范围;(ii) 酶对底物的周转时间会随着土壤湿度的降低而增加。该研究旨在描述β-葡萄糖苷酶和酸性磷酸酶在距根尖一定距离内的分布特征。此外,还调查了酶活性以及植物根和芽的特征(长度和重量)。结果表明,与根的长度相比,芽的长度受干旱的影响更大,前者减少了 25%,后者减少了 5%。同时,在干旱条件下,植株的芽重减少了 61%,根重减少了 90%。从根尖开始测量,单株植物根部的酶热点范围也随干旱而减少。此外,干旱还降低了根瘤菌酶的活性,导致β-D-吡喃葡萄糖苷(MUF-G)和 4-甲基伞形酮基磷酸(MUF-P)底物的周转时间变慢。这项研究揭示了干旱对根系与微生物相互作用的不利影响,最终导致作物生长不良。
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来源期刊
VIETNAM JOURNAL OF EARTH SCIENCES
VIETNAM JOURNAL OF EARTH SCIENCES GEOSCIENCES, MULTIDISCIPLINARY-
CiteScore
3.60
自引率
20.00%
发文量
0
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