Beta-carbonic anhydrase gene expression levels change depending on the drought severity in both the leaves and roots of Arabidopsis thaliana

IF 1.5 4区生物学 Q3 PLANT SCIENCES

Turkish Journal of Botany Pub Date : 2023-11-22 DOI:10.55730/1300-008x.2783

Sertan Çevik, Oğuzhan Kurt, Ayşi̇n GÜZEL DEĞER, F. Kocacinar, Riza Binzet

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引用次数: 0

Abstract

: In this study, it was aimed to determine the relationship between β carbonic anhydrase (βCA) gene expression levels and drought stress severity. For this purpose, the expression levels of six βCA genes in the roots and leaves of Arabidopsis thaliana were investigated under mild, moderate, and severe drought conditions. In addition, changes in the biomass, leaf water content, photosynthetic efficiency, CA enzyme activity, free proline content, and lipid peroxidation were also determined. The results showed that all of the β CA gene expression levels and CA enzyme activity increased in the leaf under mild drought conditions; however, the expression levels (except for βCA-6) and enzyme activity decreased as the severity of drought increased. In the roots, the gene expression levels were very low compared to the leaves and all of the βCA gene expressions decreased under drought stress. Moreover, depending on the severity of the drought stress, the biomass, leaf water content, and photosynthetic efficiency decreased, while the content of proline and lipid peroxidation increased. A positive correlation was determined between the photosynthetic efficiency and high βCA expression level. The results indicated that βCA genes may play important roles, especially in the onset of drought stress in A. thaliana . When the findings were evaluated together, βCA genes may be considered to be important candidate genes for increasing or maintaining photosynthetic yield of C 3 plants under drought stress conditions.

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拟南芥叶片和根部的β-碳酸酐酶基因表达水平随干旱严重程度而变化

:本研究旨在确定β碳酸酐酶（βCA）基因表达水平与干旱胁迫严重程度之间的关系。为此，研究了拟南芥在轻度、中度和重度干旱条件下根和叶中六个 βCA 基因的表达水平。此外，还测定了拟南芥的生物量、叶片含水量、光合效率、CA酶活性、游离脯氨酸含量和脂质过氧化反应的变化。结果表明，在轻度干旱条件下，叶片中所有 β CA 基因的表达水平和 CA 酶活性都有所增加；但随着干旱程度的增加，表达水平（除 βCA-6 外）和酶活性都有所下降。与叶片相比，根部的基因表达水平很低，在干旱胁迫下，所有 βCA 基因的表达量都有所下降。此外，根据干旱胁迫的严重程度，生物量、叶片含水量和光合效率下降，而脯氨酸含量和脂质过氧化物增加。光合效率与高βCA表达水平呈正相关。结果表明，βCA 基因可能发挥了重要作用，尤其是在旱胁迫开始时。综合上述研究结果，βCA 基因可能被认为是在干旱胁迫条件下提高或维持 C 3 植物光合产量的重要候选基因。

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来源期刊

Turkish Journal of Botany PLANT SCIENCES-

CiteScore

2.90

自引率

5.60%

发文量

审稿时长

6-12 weeks

期刊介绍： The Turkish Journal of Botany is published electronically 6 times a year by the Scientific and Technological Research Council of Turkey (TÜBİTAK) and accepts manuscripts (in English) covering all areas of plant biology (including genetics, evolution, systematics, structure, function, development, diversity, conservation biology, biogeography, paleobotany, ontogeny, functional morphology, ecology, reproductive biology, and pollination biology), all levels of organisation (molecular to ecosystem), and all plant groups and allied organisms (algae, fungi, and lichens). Authors are required to frame their research questions and discuss their results in terms of major questions in plant biology. In general, papers that are too narrowly focused, purely descriptive, or broad surveys, or that contain only preliminary data or natural history, will not be considered (*). The following types of article will be considered: 1. Research articles: Original research in various fields of botany will be evaluated as research articles. 2. Research notes: These include articles such as preliminary notes on a study or manuscripts on the morphological, anatomical, cytological, physiological, biochemical, and other properties of plant, algae, lichen and fungi species. 3. Reviews: Reviews of recent developments, improvements, discoveries, and ideas in various fields of botany. 4. Letters to the editor: These include opinions, comments relating to the publishing policy of the Turkish Journal of Botany, news, and suggestions. Letters should not exceed one journal page. (*) 1. Raw floristic lists (of algae, lichens, fungi, or plants), species descriptions, chorological studies, and plant sociology studies without any additional independent approaches. 2. Comparative morphology and anatomy studies (that do not cover a family, tribe, subtribe, genus, subgenus, section, subsection, or species complexes with taxonomical problems) without one or more independent additional approaches such as phylogenetical, micromorphological, chromosomal and anatomical analyses. 3. Revisions of family, tribe, genus, subgenus, section, subsection, or species complexes without any original outputs such as taxonomical status changes, IUCN categories, and phenological and ecological analyses. 4. New taxa of all plants without any additional independent approaches such as phylogenetical, ecological, chromosomal, chorological and correlational analyses in addition to a detailed macro- and micro-morphological descriptions with quality field and microscopic illustrations of taxonomically important structures and identification key in the taxonomic group. New records of all plants without any additional independent approaches such as phylogenetical, ecological, chromosomal, chorological and correlational analyses in addition to a detailed macro- and micro-morphological descriptions with quality field and microscopic illustrations of taxonomically important structures and identification key in the taxonomic group may be accepted for peer review if they contain 3 or more new records or taxonomical status update, such as lectotypification, new combinations, transfers, revivals and synonyms. 5. New taxa of algae, lichens, and fungi without any additional independent approaches such as phylogenetical, ecological, chromosomal, chorological and correlational analyses in addition to a detailed macro- and micro-morphological descriptions with quality field and microscopic illustrations of taxonomically important structures and identification key in the taxonomic group. New records of algae, lichens, and fungi without any additional independent approaches such as phylogenetical, ecological, chromosomal, chorological and correlational analyses in addition to a detailed macro- and micro-morphological descriptions with quality field and microscopic illustrations of taxonomically important structures and identification key in the taxonomic group may be accepted for peer review if they contain 5 or more new records or taxonomical status update, such as lectotypification, new combinations, transfers, revivals and synonyms.