Transcriptome Analysis of the Cultured Hybrid Grouper (♀Epinephelus fuscoguttatus×♂E. lanceolatus) Immunized with Vibrio harveyi formalin-killed cells vaccine (FKC) combined with chitosan oligosaccharide

Israeli Journal of Aquaculture - Bamidgeh Pub Date : 2023-11-27 DOI:10.46989/001c.88370

Xiao-jun Wan, Fan Da, Guixiang Lin, Yucong Huang, S. Cai, Jichang Jian

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Abstract

Grouper has become an essential mariculture species in China, while vibriosis caused by Vibrio harveyi significantly impacts its culture. Our previous study confirmed the V. harveyi formalin-killed cells vaccine (FKC) combined with chitosan oligosaccharide (FKC+COS) effectively prevents vibriosis. As an adjuvant, COS could significantly enhance FKC effectiveness against V. harveyi in grouper. In the present study, we performed transcriptome analysis of grouper spleens tissue 14 days post-immunization of PBS and FKC+COS, respectively. After assembly and annotation, 2,503 differentially expressed genes (DEGs) were obtained, including the upregulated 1,894 DEGs and downregulated 609 DEGs between the PBS group and FKC+COS group. To explore the relevance of DEGs in immunity, enrichment analysis in the KEGG database revealed that the main pathways of DEGs distribution associated with immunity were antigen processing and presentation, lysosome, the intestinal immune network for IgA production and FcγR-mediated phagocytosis. In conclusion, transcriptome analysis of spleens was performed to explore the potential mechanism of COS as an adjuvant enhancing the protection effectiveness of FKC against vibriosis in grouper.

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用结合壳聚糖寡糖的哈维氏弧菌福尔马林灭活细胞疫苗（FKC）免疫杂交石斑鱼（♀Epinephelus fuscoguttatus×♂E.

石斑鱼已成为中国重要的海水养殖品种，而由哈维氏弧菌引起的弧菌病严重影响了石斑鱼的养殖。我们之前的研究证实，福尔马林杀灭细胞疫苗（FKC）与壳聚糖低聚糖（FKC+COS）结合使用可有效预防弧菌病。作为一种佐剂，COS 能显著提高 FKC 对石斑鱼中 Harveyi 病毒的效果。在本研究中，我们分别对PBS和FKC+COS免疫后14天的石斑鱼脾脏组织进行了转录组分析。经过组装和注释，我们得到了 2503 个差异表达基因（DEGs），其中包括 PBS 组和 FKC+COS 组之间上调的 1894 个 DEGs 和下调的 609 个 DEGs。为了探讨 DEGs 在免疫中的相关性，在 KEGG 数据库中进行了富集分析，发现 DEGs 的分布与免疫相关的主要通路为抗原加工和递呈、溶酶体、肠道免疫网络产生 IgA 和 FcγR 介导的吞噬作用。总之，对脾脏进行转录组分析是为了探索 COS 作为一种佐剂提高 FKC 对石斑鱼弧菌病的保护效果的潜在机制。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Israeli Journal of Aquaculture - Bamidgeh

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