Purification of the apple pomace polyphenol extract using adsorbent resin

Q3 Agricultural and Biological Sciences

Progress in Agricultural Engineering Sciences Pub Date : 2023-11-28 DOI:10.1556/446.2023.00087

Faraja Gonelimali, B. Szabó-Nótin, M. Máté

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引用次数: 0

Abstract

Polyphenols from agro-industrial waste particularly of fruit origin are a reliable source of antioxidants and antimicrobials that can be used as natural food additives. Organic solvents play an important role in extracting the polyphenols, however, inefficiency in exerting bioactivity and interference with the organoleptic properties are among the reasons that hinder their use as food additives. These problems can be alleviated by purification. In this study, the effect of resin types and elution solvent for purification of the apple pomace extracts on total phenolic content (TPC) and antioxidants were investigated. Crude ethanolic extracts were purified using amberlite resins (XAD7HP and FPX66) in a glass column (25 × 310 mm). The sorption flow rate was 2 Bed volume (BV) per hour, rinse 2 BV per hour, and desorption was 2 BV per hour. Final wash and regeneration were each done by 2 BV per hour. Polyphenol content and antioxidant capacity were quantified spectrophotometrically using Folin-Ciocalteu and Ferric reducing ability of plasma (FRAP) assays respectively. Polyphenol recovery was 50% in XAD7HP (Lowest) using ethanol and 69% in FPX66 (Highest) using acetone. For the case of FRAP recovery, 76% (Lowest) was observed in FPX66 using ethanol while 93% (Highest) was observed in XAD7HP using acetone. Conclusively, FPX66 is the ideal resin for the purification of apple pomace extracts for enhancing antioxidant activity compared to XAD7HP. Further, acetone seems to be a good desorption solvent compared to ethanol.

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利用吸附树脂提纯苹果渣多酚提取物

从农用工业废弃物（尤其是水果）中提取的多酚是抗氧化剂和抗菌剂的可靠来源，可用作天然食品添加剂。有机溶剂在提取多酚过程中发挥着重要作用，但是，有机溶剂在发挥生物活性方面效率低下，而且会干扰感官特性，这些都是阻碍将多酚用作食品添加剂的原因。这些问题都可以通过提纯得到缓解。本研究考察了纯化苹果渣提取物的树脂类型和洗脱溶剂对总酚含量（TPC）和抗氧化剂的影响。在玻璃柱（25 × 310 毫米）中使用琥珀酸树脂（XAD7HP 和 FPX66）纯化粗乙醇提取物。吸附流速为每小时 2 BV，冲洗流速为每小时 2 BV，解吸流速为每小时 2 BV。最后的洗涤和再生各为每小时 2 BV。多酚含量和抗氧化能力分别用 Folin-Ciocalteu 和血浆铁还原能力（FRAP）测定法进行分光光度定量。使用乙醇对 XAD7HP（最低）的多酚回收率为 50%，使用丙酮对 FPX66（最高）的多酚回收率为 69%。在 FRAP 回收率方面，FPX66 使用乙醇的回收率为 76%（最低），而 XAD7HP 使用丙酮的回收率为 93%（最高）。因此，与 XAD7HP 相比，FPX66 是纯化苹果渣提取物以提高抗氧化活性的理想树脂。此外，与乙醇相比，丙酮似乎是一种很好的解吸溶剂。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Progress in Agricultural Engineering Sciences Engineering-Industrial and Manufacturing Engineering

CiteScore

1.80

自引率

0.00%

发文量

期刊介绍： The Journal publishes original papers, review papers and preliminary communications in the field of agricultural, environmental and process engineering. The main purpose is to show new scientific results, new developments and procedures with special respect to the engineering of crop production and animal husbandry, soil and water management, precision agriculture, information technology in agriculture, advancements in instrumentation and automation, technical and safety aspects of environmental and food engineering.