A Novel HPLC Method for Rapid Determination of HMF in Dulce de Leche: A Potential Tool for Quality Control

Journal of Physical Chemistry and Functional Materials Pub Date : 2023-11-29 DOI:10.54565/jphcfum.1392507

S. Şanlı, Halil Ozan Saraç

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Abstract

A validated isocratic method utilizing reverse-phase high-performance liquid chromatography (RP-HPLC) was developed for the quantification of hydroxymethylfurfural (HMF) in five distinct commercially available Dulce de Leche samples that are sold in Turkish markets. The X-Terra RP-18 column, which has dimensions of 150 × 4.60 mm i.d. and a particle size of 5 μm, was selected for the stationary phase. The separation process was conducted isocratically by utilizing a methanol-water mixture (10:90, v/v) containing 1% TCA as the mobile phase. The separation was carried out at a temperature of 25 oC and a flow rate of 0.5 mL/min. The detection was performed at a wavelength of 284 nm. The methodology exhibited favorable selectivity and linearity, as evidenced by a correlation coefficient (r) exceeding 0.999. The obtained values for the limit of detection (LOD) and limit of quantification (LOQ) were 0.0019 μg/mL and 0.0059 μg/mL, respectively. Additionally, the recovery rate was determined to be 95.45%. Furthermore, it has been reported that the levels of HMF in commercially available Dulce de Leche originating from Turkey vary between 50.55 and 90.18 mg/kg for the total HMF content.

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快速测定奶昔中 HMF 的新型 HPLC 方法：一种潜在的质量控制工具

利用反相高效液相色谱法（RP-HPLC）开发了一种有效的等度方法，用于定量检测土耳其市场上销售的五种不同市售果仁乳酪样品中的羟甲基糠醛（HMF）。固定相选用了 X-Terra RP-18 色谱柱，其内径为 150 × 4.60 mm，粒径为 5 μm。分离过程以含 1%三氯乙酸的甲醇-水混合物（10:90，v/v）为流动相进行等压分离。分离温度为 25 oC，流速为 0.5 mL/min。检测波长为 284 nm。该方法具有良好的选择性和线性，相关系数（r）超过 0.999。检测限（LOD）和定量限（LOQ）分别为 0.0019 μg/mL 和 0.0059 μg/mL。此外，测定的回收率为 95.45%。此外，据报道，土耳其市售 Dulce de Leche 中的 HMF 总含量介于 50.55 至 90.18 毫克/千克之间。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Journal of Physical Chemistry and Functional Materials

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