The effect of topical 0.5% humic acid gel on male rats with skin ulcer

Q3 Medicine

Journal of Cutaneous and Aesthetic Surgery Pub Date : 2023-11-30 DOI:10.4103/jcas.jcas_104_23

Fatemeh Samiee-Rad, Fatemeh Ghasemi, Ensiyeh Bahadoran, M. Sofiabadi, Zahra Shalbaf, Armane Taherkhani, N. Gheibi

{"title":"The effect of topical 0.5% humic acid gel on male rats with skin ulcer","authors":"Fatemeh Samiee-Rad, Fatemeh Ghasemi, Ensiyeh Bahadoran, M. Sofiabadi, Zahra Shalbaf, Armane Taherkhani, N. Gheibi","doi":"10.4103/jcas.jcas_104_23","DOIUrl":null,"url":null,"abstract":"Humic derivatives have antibacterial and anti-inflammatory properties. This study aimed to assess the experimental wound-healing effect of 0.5% humic acid gel. A full-thickness skin wound was created on the dorsal side of 24 Sprague Dawley male rats (220–250g). The animals were then randomly divided into the control, sham, and experimental groups. Skin wounds were bandaged daily using sterile gauze dipped in normal saline, carboxymethylcellulose, and 0.5% humic acid for 21 days. The wound-healing rate was evaluated grossly and histologically at various time intervals post-injury. Wound-healing percentage was significantly higher in the gel treatment group at all time points (P < 0.05). The mean number of inflammatory cells was significantly lower in the humic acid gel group than in the other groups (P < 0.001). Moreover, the number of new vascular cells and fibroblasts were significantly increased in the humic acid gel compared to the control (P < 0.001). These data confirmed that 0.5% humic acid gel accelerates wound healing, probably by anti-inflammatory effects, as well as by promoting vascular and fibroblast proliferation. Therefore, the humic acid gel may be used to improve wound care.","PeriodicalId":15415,"journal":{"name":"Journal of Cutaneous and Aesthetic Surgery","volume":"380 ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2023-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Cutaneous and Aesthetic Surgery","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.4103/jcas.jcas_104_23","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Medicine","Score":null,"Total":0}

引用次数: 0

Abstract

Humic derivatives have antibacterial and anti-inflammatory properties. This study aimed to assess the experimental wound-healing effect of 0.5% humic acid gel. A full-thickness skin wound was created on the dorsal side of 24 Sprague Dawley male rats (220–250g). The animals were then randomly divided into the control, sham, and experimental groups. Skin wounds were bandaged daily using sterile gauze dipped in normal saline, carboxymethylcellulose, and 0.5% humic acid for 21 days. The wound-healing rate was evaluated grossly and histologically at various time intervals post-injury. Wound-healing percentage was significantly higher in the gel treatment group at all time points (P < 0.05). The mean number of inflammatory cells was significantly lower in the humic acid gel group than in the other groups (P < 0.001). Moreover, the number of new vascular cells and fibroblasts were significantly increased in the humic acid gel compared to the control (P < 0.001). These data confirmed that 0.5% humic acid gel accelerates wound healing, probably by anti-inflammatory effects, as well as by promoting vascular and fibroblast proliferation. Therefore, the humic acid gel may be used to improve wound care.

查看原文本刊更多论文

外用 0.5% 腐植酸凝胶对雄性皮肤溃疡大鼠的影响

腐植酸衍生物具有抗菌消炎的特性。本研究旨在评估 0.5% 腐植酸凝胶的伤口愈合效果。在 24 只 Sprague Dawley 雄性大鼠（220-250 克）的背侧创建一个全厚皮肤伤口。然后将动物随机分为对照组、假组和实验组。每天用蘸有生理盐水、羧甲基纤维素和 0.5% 腐植酸的无菌纱布包扎皮肤伤口，持续 21 天。在受伤后的不同时间间隔，对伤口愈合率进行大体和组织学评估。在所有时间点上，凝胶处理组的伤口愈合率都明显更高（P < 0.05）。腐植酸凝胶组的炎症细胞平均数量明显低于其他组（P < 0.001）。此外，与对照组相比，腐植酸凝胶组新生血管细胞和成纤维细胞的数量明显增加（P < 0.001）。这些数据证实，0.5% 腐植酸凝胶可加速伤口愈合，这可能是通过抗炎作用以及促进血管和成纤维细胞增殖实现的。因此，腐植酸凝胶可用于改善伤口护理。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Cutaneous and Aesthetic Surgery Medicine-Surgery

CiteScore

1.10

自引率

0.00%

发文量

审稿时长

29 weeks

期刊介绍： The journal will cover aspects of Dermatosurgery and Aesthetic surgery, including cutaneous surgical procedures, lasers, light based treatments and aesthetic treatments. Authors of articles addressing topics such as skin tumours, pigmentary disorders, histopathology, diagnostic techniques, drugs, applications of cosmeceuticals surgical aspects, including pre and post procedural care are highly encouraged to submit with us. The journal also publishes articles on topics in allied specialities suh as plastic surgery, maxillofacial surgery vascular surgery, oculoplastic surgery etc. that are relevant to Dermatosurgery. The journal gives preference to clinically oriented studies over experimental and animal studies. The Journal would publish peer-reviewed Original Research Articles, Systematic reviews, Narrative Reviews, Case reports, Innovations and Debates. Letters to the editor, practice points, pearls in cutaneous and aesthetic surgery, quiz page, images, novel techniques, newer uses of instruments and video presentations and ethical aspects of cutaneous and aesthetic surgery are also invited.