METABOLIT SEKUNDER DARI FRAKSI AKTIF AKAR PANDAN (Pandanus amaryllifolius roxb.) YANG BERSIFAT ANTIFEEDANT TERHADAP Epilachna sparsa (SECONDARY METABOLITES OF THE ACTIVE FRACTION OF PANDAN ROOT (Pandanus amaryllifolius roxb.) AS ANTIFEEDANT AGAINST Epilachna sparsa)

Ari Widiyantoro, Harlia Harlia, Annisa Dyah Astari Putri
{"title":"METABOLIT SEKUNDER DARI FRAKSI AKTIF AKAR PANDAN (Pandanus amaryllifolius roxb.) YANG BERSIFAT ANTIFEEDANT TERHADAP Epilachna sparsa (SECONDARY METABOLITES OF THE ACTIVE FRACTION OF PANDAN ROOT (Pandanus amaryllifolius roxb.) AS ANTIFEEDANT AGAINST Epilachna sparsa)","authors":"Ari Widiyantoro, Harlia Harlia, Annisa Dyah Astari Putri","doi":"10.26418/indonesian.v6i3.71213","DOIUrl":null,"url":null,"abstract":"Pandan wangi (Pandanus amaryllifolius Roxb.) contains flavonoids, terpenoids, steroids, saponins, alkaloids and tannins which can be used as antifeedant compounds. This study aims to determine the antifeedant activity and to identify of secondary metabolites from pandan roots extract against Epilachna sparsa. The antifeedant activity test was carried out on the methanol extract and the partition yeild fraction. The research was carried out by maceration of pandan root which had been mashed into powder with methanol solution to obtain a methanol extract of 86.51 g. The extract was then separated by partitioning to obtain methanol fraction, ethyl acetate fraction, dichloromethane fraction and n-hexane fraction. The methanol extract and fraction from partition results were then tested for antifeedant activity against Epilachna sparsa. The results showed that at a concentration of 6% the methanol extract had an antifeedant activity of 59.37 ± 2.11 % against Epilachna sparsa. The fractions resulting from the partition included methanol, ethyl acetate, dichloromethane and n-hexane fractions at a concentration of 6% respectively showing antifeedant activity of 81.25 ± 3.56 %; 75.00 ± 2.99 %; 37.50 ± 3.77 % and 21.18 ±1.23 %. Based on the antifeedant activity test results, the methanol fraction showed the best activity. Furthermore, the methanol fraction was separated and purified by chromatographic techniques. The isolate obtained was 53.11 mg in the form of white needle crystals and had a melting point of 135-138oC. The isolate based on the phytochemical test showed positive for flavonoid","PeriodicalId":13554,"journal":{"name":"Indonesian Journal of Pure and Applied Chemistry","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Indonesian Journal of Pure and Applied Chemistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.26418/indonesian.v6i3.71213","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Pandan wangi (Pandanus amaryllifolius Roxb.) contains flavonoids, terpenoids, steroids, saponins, alkaloids and tannins which can be used as antifeedant compounds. This study aims to determine the antifeedant activity and to identify of secondary metabolites from pandan roots extract against Epilachna sparsa. The antifeedant activity test was carried out on the methanol extract and the partition yeild fraction. The research was carried out by maceration of pandan root which had been mashed into powder with methanol solution to obtain a methanol extract of 86.51 g. The extract was then separated by partitioning to obtain methanol fraction, ethyl acetate fraction, dichloromethane fraction and n-hexane fraction. The methanol extract and fraction from partition results were then tested for antifeedant activity against Epilachna sparsa. The results showed that at a concentration of 6% the methanol extract had an antifeedant activity of 59.37 ± 2.11 % against Epilachna sparsa. The fractions resulting from the partition included methanol, ethyl acetate, dichloromethane and n-hexane fractions at a concentration of 6% respectively showing antifeedant activity of 81.25 ± 3.56 %; 75.00 ± 2.99 %; 37.50 ± 3.77 % and 21.18 ±1.23 %. Based on the antifeedant activity test results, the methanol fraction showed the best activity. Furthermore, the methanol fraction was separated and purified by chromatographic techniques. The isolate obtained was 53.11 mg in the form of white needle crystals and had a melting point of 135-138oC. The isolate based on the phytochemical test showed positive for flavonoid
METABOLIT SEKUNDER DARI FRAKSI AKTIF AKAR PANDAN (Pandanus amaryllifolius roxb.) YANG BERSIFAT ANTIFEEDANT TERHADAP Epilachna sparsa (SECONDARY METABOLITES OF THE ACTIVE FRACTION OF PANDAN ROOT (Pandanus amaryllifolius roxb.) AS ANTIFEEDANT AGAINST Epilachna sparsa)
Pandan wangi(Pandanus amaryllifolius Roxb.)含有黄酮类、萜类、类固醇、皂苷、生物碱和单宁酸,这些物质可用作抗寄生虫化合物。本研究旨在确定丹参根提取物对 Epilachna sparsa 的抗飞虫活性,并鉴定其中的次生代谢物。对甲醇提取物和分配酵母部分进行了抗飞虫活性测试。研究方法是用甲醇溶液浸泡捣碎成粉末的板蓝根,得到 86.51 克的甲醇提取物,然后通过分馏分离得到甲醇馏分、乙酸乙酯馏分、二氯甲烷馏分和正己烷馏分。然后,对甲醇提取物和分馏结果中的馏分进行了针对 Epilachna sparsa 的抗食蚜蝇活性测试。结果表明,浓度为 6% 的甲醇提取物对 Epilachna sparsa 的抗药性为 59.37 ± 2.11%。分配产生的馏分包括浓度为 6% 的甲醇、乙酸乙酯、二氯甲烷和正己烷馏分,其抗药性分别为 81.25 ± 3.56 %、75.00 ± 2.99 %、37.50 ± 3.77 % 和 21.18 ± 1.23 %。根据抗飞虫活性测试结果,甲醇馏分显示出最佳活性。此外,还利用色谱技术对甲醇馏分进行了分离和纯化。获得的分离物为 53.11 毫克白色针状晶体,熔点为 135-138 摄氏度。根据植物化学测试,分离物的黄酮类化合物呈阳性反应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信