Effects of Extraction Temperature on Polyphenol Compounds and Antioxidant Activity of Golden Bladderwort (Utricularia aurea)

IF 0.2 Q4 AGRONOMY
agriTECH Pub Date : 2023-11-30 DOI:10.22146/agritech.75223
S. Sudirman, A. Baehaki, Fajar Fathullah, Miftahul Janna
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Abstract

The excess of free radicals is known to induce dysfunction in tissues and cells within the human body. This phenomenon is caused by the imbalance between antioxidants and free radical production necessitating the need for exogenous antioxidants. Golden bladderwort ( Utricularia aurea ) plant may have the potential as a source of antioxidant. Therefore, this study aimed to investigate the effect of extraction temperature on the polyphenol compounds and the antioxidant activity of the golden bladderwort plant. The dried powder from the plant was extracted using 70% ethanol at 30, 45 and 60 ºC, with each treatment repeated three times. The antioxidant activity was analyzed by using the 2,2-diphenyl-1-picrylhydrazyl method. The results showed that the yield of extraction was 1.34% (30 ºC), 4.00% (45 ºC) and 12.48% (60 ºC). The total polyphenol and flavonoid contents found at 60ºC were 50.80 mg QE/g dried sample and 60.01 mg QE/g dried sample, respectively. Furthermore, the antioxidant activity was calculated in half-maximum inhibitory concentration (IC 50 ), and the values obtained were 0,38 mg/mL (45 ºC), 0,35 mg/mL (30 ºC) and 0,11 mg/mL (60 ºC). These results indicated that temperature of extraction affected the level of polyphenol contents and the antioxidant activity of the golden bladderwort extract. The high extraction temperature (60 ºC) emerged as the most optimal condition for extraction with effective antioxidant activity. Therefore, the results can be used as a reference for future investigations involving the extraction of polyphenol compounds from golden bladderwort as a source of natural antioxidants.
提取温度对金膀胱草(Utricularia aurea)多酚化合物和抗氧化活性的影响
众所周知,过量的自由基会导致人体内的组织和细胞功能失调。这种现象是由抗氧化剂和自由基产生之间的不平衡造成的,因此需要外源性抗氧化剂。金膀胱草(Utricularia aurea)可能具有抗氧化剂来源的潜力。因此,本研究旨在探讨提取温度对金膀胱草多酚化合物和抗氧化活性的影响。在 30、45 和 60 ºC 的温度下,用 70% 的乙醇萃取该植物的干燥粉末,每个处理重复三次。抗氧化活性采用 2,2-二苯基-1-苦基肼法进行分析。结果表明,提取率分别为 1.34%(30 ºC)、4.00%(45 ºC)和 12.48%(60 ºC)。在 60ºC 时,多酚和类黄酮的总含量分别为 50.80 毫克 QE/克干燥样品和 60.01 毫克 QE/克干燥样品。此外,抗氧化活性以半最大抑制浓度(IC 50)计算,得到的值分别为 0.38 毫克/毫升(45 ºC)、0.35 毫克/毫升(30 ºC)和 0.11 毫克/毫升(60 ºC)。这些结果表明,萃取温度会影响金囊草提取物的多酚含量水平和抗氧化活性。高萃取温度(60 ºC)是具有有效抗氧化活性的最佳萃取条件。因此,该结果可作为今后从金囊草中提取多酚化合物作为天然抗氧化剂来源的研究参考。
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agriTECH
agriTECH AGRONOMY-
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审稿时长
24 weeks
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