Cell Culture Media Exchange Automation for Screw Cap Flasks within Incubators

Abstract

The study presents a new system for automating media exchange in cell cultures. This system is designed to fit inside common incubators and uses T75 flasks as growth vessels. It follows a step-by-step process, involving rotating and tilting mechanisms to drain old media, cleanse flask interiors, and refill with fresh media. The system's design incorporates a loading platform for four flasks, driven by a stepper motor and gear mechanism. Tilting is controlled by a servo motor, while a peristaltic pump refills with fresh media. Electronics, including an Arduino Nano, are sealed for protection. Sensors ensure flask presence and media condition. Validation involved 200 water-based cycles and successful fibroblast cell culture for eight days. The system shows potential for streamlining cell maintenance, although long-term durability and contamination concerns require further investigation.