Evaluation of the content of ceruloplasmin, copper and coppercoordinating amino acids in cow milk at different lactation periods

O. Voronina, S. Zaitsev, A. Savina, N. Kolesnik
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Abstract

The purpose of the research was to study the content of ceruloplasmin, copper and copper-coordinating amino acids (in the molecule of the studied protein) in the milk of white-and- black cows at different periods of lactation. The research methods were: modified method for determining ceruloplasmin by Revin; copper was determined using a ZEEnit 650 P atomic adsorption spectrometer (Analytik Jena AG, Germany), amino acid analysis was performed using a highly efficient LC-20 Prominence liquid chromatography system (Shimadzu, Tokyo, Japan) equipped with a reaction module for post-column derivatization with ninhydrin ARM-1000 (Sevko & Co., Moscow, Russia). The study groups were formed depending on the timing of lactation: 1-2 months – group 1; 3-5 months – group 2; 6-7 months – group 3; 8-9 months – group 4. Each group included 8 animals. The Mann-Whitney U criterion and Pearson correlations were used for statistical processing of the obtained results. The average values of ceruloplasmin levels in the groups were 0.44-0.49 mg/ml, copper 71-83 mcg/l, histidine 0.11-013 g/100g, cysteine 0.03 g/100g and methionine 0.09-0.11 g/100g. In these 4 groups, small differences were found in the content of ceruloplasmin, copper and three amino acids, but only for the second and fourth groups of histidine, these differences were significant. Thus, the indicated intervals of ceruloplasmin and copper content can serve as important guidelines for determining the "reference intervals" for cow milk in general, while it is not necessary to take into account different lactation periods.
不同泌乳期牛奶中脑磷脂蛋白、铜和铜配位氨基酸含量的评估
研究的目的是研究白牛和黑牛不同泌乳期牛奶中脑磷脂蛋白、铜和铜配位氨基酸(在所研究蛋白质的分子中)的含量。研究方法是:改良雷文测定脑磷脂的方法;使用 ZEEnit 650 P 原子吸附光谱仪(Analytik Jena AG,德国)测定铜;使用高效 LC-20 Prominence 液相色谱系统(Shimadzu,日本东京)进行氨基酸分析,该系统配备茚三酮 ARM-1000 柱后衍生反应模块(Sevko & Co.,俄罗斯莫斯科)。根据泌乳时间划分研究组:每组 8 只动物。对所得结果采用曼-惠特尼 U 标准和皮尔逊相关性进行统计处理。各组的脑磷脂含量平均值为 0.44-0.49 毫克/毫升,铜 71-83 微克/升,组氨酸 0.11-013 克/100 克,半胱氨酸 0.03 克/100 克,蛋氨酸 0.09-0.11 克/100 克。在这 4 组中,脑磷脂、铜和三种氨基酸的含量差异较小,但只有第二组和第四组组氨酸的含量差异显著。因此,脑磷脂和铜含量的指示区间可作为确定一般牛奶 "参考区间 "的重要准则,而不必考虑不同的泌乳期。
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