Effect Photodynamic therapy (PDT) And Single Walled Carbon Nanotube- OH on The Skin Cancer A431 Cell line

Abstract

The aim of this in vitro study was to analyze the effect of a blue light (λ = 420 – 480 nm), a single-walled carbon nanotube -OH, and their combination on the viability (cytotoxic or enhancement) of the skin cancer A431 cell line after 24 hours of incubation periods. Cell culture is the process of removing cells from living tissues and growing them in a laboratory setting until they are ready to be tested using photodynamic therapy and nanoparticles, which is known as direct irradiation in vitro. Skin cell plates (A431 cell line) grown in culture medium supplemented with 10% fetal bovine serum (FBS) were irradiated with blue light, treated with the nanoparticle, and treated with the combination of them, and plates were incubated for 24 hours. In all the experiments, a crystal violet assay was used to determine the viability of the cells, and the intensity of color was measured by a plate reader. The cells were kept at 37°C. Blue light results showed a considerable decrease (p≤0.001) at 240 seconds after 24 hours of incubation time in viability percent; nanoparticle results showed a considerable decrease in the viability percent  (p≤0.001) for all concentrations; the most effective concentration was 200 µg/ml; and the combination results showed a significant decrease in cell viability percent (p≤0.001)  for all concentrations as compared with the control group. The single-walled carbon nanotube- OH with a concentration of 200 µg/ml produced the greatest results when combined with a light exposure period of 240 seconds.