Cyclosporine A Concentrations in Blood Measured with the Immunoassays on Roche e601® and ADVIA Centaur XP® Analysers-What is the Extent of the Agreement?

IF 0.2 Q4 CHEMISTRY, MULTIDISCIPLINARY
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Abstract

Monitoring of cyclosporine A (Cs A) concentrations is inevitable for efficient and safe immunosuppression. Currently, immunoassays are the most often used method. The study compared the Cs A concentrations in EDTA-blood samples of 50 patients, measured on Roche e601® and ADVIA Centaur XP® analyzers. The Cs A concentrations on e601® were between 30.00 and 573.00 ng/mL. On Centaur XP® they were in the range 30.2-395.2 ng/mL. For all data the correlation coefficient (95% confidence interval (CI)) was 0.98 (0.97-0.99), while in the groups with concentrations below and above 100 ng/mL it was 0.90 (0.74-0.93) and 0.98 (0.94-0.99), respectively. The slope (95% CI) in the Passing-Bablok analysis on all results was 0.73 (0.67-0.83), and the intercept (95% CI) was 12.53 (6.66-17.78). In the group with results below 100 ng/mL, the slope was 0.92 (0.77-1.12) and the intercept 3.05 (from -8.45 to 12.09). For the Cs A concentrations above 100 ng/mL the slope was 0.71 (0.64-0.84) and the intercept 9.31 (from -8.86 to 24.27). The proportional and systematic errors were present in a wide range of Cs A concentrations measured on the evaluated analyzers. The concordance was satisfactory for concentrations below 100 ng/mL.
使用罗氏 e601® 和 ADVIA Centaur XP® 分析仪上的免疫测定法测量血液中的环孢素 A 浓度--协议的范围有多大?
监测环孢素 A(Cs A)的浓度对于高效、安全的免疫抑制是必不可少的。目前,免疫测定是最常用的方法。该研究比较了用罗氏 e601® 和 ADVIA Centaur XP® 分析仪测量的 50 名患者 EDTA 血液样本中的 Cs A 浓度。e601® 的 Cs A 浓度在 30.00 至 573.00 纳克/毫升之间。Centaur XP® 上的浓度范围为 30.2-395.2 纳克/毫升。所有数据的相关系数(95% 置信区间 (CI))为 0.98(0.97-0.99),而浓度低于和高于 100 纳克/毫升的组的相关系数分别为 0.90(0.74-0.93)和 0.98(0.94-0.99)。对所有结果进行的 Passing-Bablok 分析的斜率(95% CI)为 0.73(0.67-0.83),截距(95% CI)为 12.53(6.66-17.78)。在结果低于 100 毫微克/毫升的一组中,斜率为 0.92(0.77-1.12),截距为 3.05(-8.45 至 12.09)。铯 A 浓度高于 100 毫微克/毫升时,斜率为 0.71(0.64-0.84),截距为 9.31(从-8.86 到 24.27)。在所评估的分析仪上测量的铯 A 浓度范围很广,均存在比例和系统误差。浓度低于 100 毫微克/毫升时,一致性令人满意。
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Glasnik Hemicara i Tehnologa Bosne i Hercegovine
Glasnik Hemicara i Tehnologa Bosne i Hercegovine CHEMISTRY, MULTIDISCIPLINARY-
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