Short term antibiotic effects on gut microbiome in Indian preschoolers: A 16S rRNA analysis

IF 0.9 Q3 MEDICINE, GENERAL & INTERNAL
Neha, Ashish Bavdekar, A. Kawade, K. Veligandla, Devesh Kumar Joshi, Rahul Rathod, Bhavesh P Kotak
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Abstract

Antibiotic use is associated with dysbiosis of the gut microbiome. The objective of this study is to investigate the effect of antibiotics on gut composition in children aged 3–5 years receiving antibiotics compared to children who did not receive antibiotics. A total of 54 participants aged 3–5 years were included in this multi-centric cohort study. Participants were divided into two equal groups, that is the treatment-experienced group (Group 1, n = 27, antibiotic) and the treatment-not-experienced group (Group 2, n = 27, non-antibiotic). Stool samples of study participants were collected on days 0 and 5 (± 1 day) and analyzed using 16Svedberg ribosomal ribonucleic acid (16S rRNA) gene sequencing. The Kruskal-Wallis H-test and Benjamini-Hochberg FDR correction were applied to determine the differentially abundant pathways across the zones using Statistical Analysis of Metagenomic Profiles (STAMP) (v2.1.3). A non-significant increase in the mean abundance of the Phyla Bacteroidota, Proteobacteria, Actinobacteria, and Verrucomicrobiota was observed in both groups from day 0 to day 5. An alteration in the Firmicutes/Bacteroidetes ratio was observed. A significant (P < 0.05) abundance of genus Enterobacteriaceae, Enterococcaceae, and Lactobacillaceae was observed in the participants with antibiotic treatment. The relative abundance of families Enterococcus, Lactobacillus, Sellimonas, Ruminococcus, Torques, and Eggerthella groups was observed to be significantly higher (P < 0.05) in participants with antibiotic treatment. Beta-diversity indices revealed significant differences at group and subgroup levels regarding the bacterial counts. It was observed that a short-term course of 5 days of antibiotic usage is associated with altered microbial abundance and diversity.
抗生素对印度学龄前儿童肠道微生物群的短期影响:16S rRNA 分析
抗生素的使用与肠道微生物组的菌群失调有关。本研究的目的是调查抗生素对 3-5 岁儿童肠道组成的影响,并将接受抗生素治疗的儿童与未接受抗生素治疗的儿童进行比较。 这项多中心队列研究共纳入了 54 名 3-5 岁的参与者。参与者被分为两个相同的组别,即接受治疗组(第1组,n = 27,抗生素组)和未接受治疗组(第2组,n = 27,非抗生素组)。研究人员在第 0 天和第 5 天(±1 天)采集粪便样本,并使用 16Svedberg 核糖体核糖核酸(16S rRNA)基因测序进行分析。 采用 Kruskal-Wallis H 检验和 Benjamini-Hochberg FDR 校正,使用 Metagenomic Profiles 统计分析(STAMP)(v2.1.3)确定各区差异丰富的通路。 从第 0 天到第 5 天,两组中的类杆菌科、变形菌科、放线菌科和疣状微生物科的平均丰度均无显著增加。此外,还观察到固着菌/类杆菌比例发生了变化。在接受抗生素治疗的参与者中,肠杆菌科、肠球菌科和乳酸菌科细菌的数量明显增加(P < 0.05)。在接受抗生素治疗的参与者中,肠球菌属、乳酸杆菌属、ellimonas 菌属、反刍球菌属、Torques 菌属和 Eggerthella 菌属的相对丰富度明显更高(P < 0.05)。Beta 多样性指数显示,各组和亚组的细菌数量存在显著差异。 据观察,短期使用 5 天抗生素与微生物丰度和多样性的改变有关。
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来源期刊
Journal of Laboratory Physicians
Journal of Laboratory Physicians MEDICINE, GENERAL & INTERNAL-
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发文量
99
审稿时长
31 weeks
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