Chlorogenic Acid and Biohanin A from Trifolium pratense L. Callus Culture Extract: Functional Activity In Vivo

Q3 Economics, Econometrics and Finance
I. Milentyeva, A. Vesnina, A. Fedorova, Elena Ostapova, T. Larichev
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Abstract

Polyphenols are potential neuroprotectors that increase lifespan and slow down aging. Red clover (Trifolium pratense L.) is a promising source of biologically active substances. Its extracts contain biochanin A and chlorogenic acid. This research used Caenorhabditis elegans to study the effect of polyphenols extracted from red clover callus cultures on SOD-3 and HSP-16.2 genes, as well as their anti-amyloid potential. The chlorogenic acid and biochanin A (200, 100, 50, and 10 µM) with a purity of 95% were isolated from callus extracts of T. pratense L. The effect of polyphenols on SOD-3 and HSP-16.2 was assessed after 5 and 2 h of heat stress (35°C), respectively, using C. elegans N2 Bristol as model organism. The neuroprotective potential was measured by counting paralyzed nematodes after 18, 40, and 62 h of incubation. The research established a dose-dependent effect between the concentration of biologically active substances and the percentage of paralyzed nematodes after 18 h of cultivation. The lowest paralysis phenotype count occurred at a concentration of 200 μM. The activity of 200 μM biochnin A was 1.18 times as high as that of a 200 μM chlorogenic acid solution. Biochanin A solutions increased SOD-3 expression by 3.7 times, compared to the control. The biologically active substances exhibited relative neuroprotective activity and affected the expression of antioxidant defense gene in C. elegans.
三叶草(Trifolium pratense L.)胼胝体培养提取物中的绿原酸和生物汉素 A:体内功能活性
多酚是潜在的神经保护剂,可延长寿命和延缓衰老。红三叶(Trifolium pratense L.)是一种很有前景的生物活性物质来源。其提取物中含有生物碱 A 和绿原酸。本研究利用秀丽隐杆线虫研究了从红三叶草胼胝体培养物中提取的多酚对 SOD-3 和 HSP-16.2 基因的影响及其抗淀粉样蛋白的潜力。 以 elegans N2 Bristol 为模型生物,分别评估了热胁迫(35°C)5 小时和 2 小时后多酚对 SOD-3 和 HSP-16.2 的影响。在培养 18、40 和 62 小时后,通过计算瘫痪线虫的数量来测量神经保护潜力。 研究发现,生物活性物质的浓度与培养 18 小时后瘫痪线虫的百分比之间存在剂量依赖效应。浓度为 200 μM 时,麻痹表型数量最少。200 μM 生物菌素 A 的活性是 200 μM 绿原酸溶液的 1.18 倍。与对照组相比,生物皂苷 A 溶液使 SOD-3 的表达量增加了 3.7 倍。 这些生物活性物质表现出了相对的神经保护活性,并影响了秀丽隐杆线虫体内抗氧化防御基因的表达。
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来源期刊
Food Processing: Techniques and Technology
Food Processing: Techniques and Technology Engineering-Industrial and Manufacturing Engineering
CiteScore
1.40
自引率
0.00%
发文量
82
审稿时长
12 weeks
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