Optimization of cultural parameters for lipase production by indigenous stenotrophomonas sp. And pseudomonas aeruginosa isolated from lipid-rich environments

IF 0.4 4区生物学 Q4 PLANT SCIENCES

Bangladesh Journal of Botany Pub Date : 2023-12-31 DOI:10.3329/bjb.v52i4.70580

Lovely Aktar, M. Saha

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引用次数: 0

Abstract

The present study aims to identify prospective lipase-producing bacteria and optimize different culture parameters to produce maximum lipase. A total of 37 lipase-positive bacteria were isolated from lipid-rich environments. Among them, strains S1N-2 and S10P-1 were found to have potential and were molecularly identified as Stenotrophomonas species ICMM10 and Pseudomonas aeruginosa BA7823, respectively. For maximum lipase production, the optimum pH was 7 and the temperature was 37ºC. Using these conditions, the isolates were grown on media with additional carbon and nitrogen sources along with different inoculum concentrations. The maximum amount of lipase produced by Stenotrophomonas sp. ICMM10 was 136.47 ± 1.63 U/ml, which is 5-fold higher than before. Pseudomonas aeruginosa BA7823 produced a maximum lipase of 96.33 ± 0.21 U/ml, which was about 9-fold higher than before. These results would provide useful information about lipase production by indigenous isolates, which may be employed as a potential bacterial lipase source of biotechnological interest. Bangladesh J. Bot. 52(4): 979-988, 2023 (December)

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从富含脂质的环境中分离出的本地赤霉单胞菌和铜绿假单胞菌生产脂肪酶的培养参数优化

本研究旨在确定潜在的脂肪酶产生细菌，并优化不同的培养参数，以产生最大限度的脂肪酶。研究人员从富含脂质的环境中分离出 37 种脂肪酶阳性细菌。其中，S1N-2 和 S10P-1 菌株具有潜力，经分子鉴定，这两株菌株分别为 Stenotrophomonas species ICMM10 和 Pseudomonas aeruginosa BA7823。为了最大限度地产生脂肪酶，最佳 pH 值为 7，温度为 37ºC。在这些条件下，分离物在添加了碳源和氮源的培养基上以不同的接种浓度进行生长。ICMM10 产生的最大脂肪酶量为 136.47 ± 1.63 U/ml ，比以前高出 5 倍。铜绿假单胞菌 BA7823 产生的最大脂肪酶为 96.33 ± 0.21 U/ml ，比以前高出约 9 倍。这些结果将提供有关本地分离物产生脂肪酶的有用信息，可用作具有生物技术价值的潜在细菌脂肪酶来源。孟加拉国植物学杂志52(4):979-988，2023 年（12 月）

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来源期刊

Bangladesh Journal of Botany 生物-植物科学

CiteScore

0.80

自引率

0.00%

发文量

审稿时长

9 months

期刊介绍： Bangladesh in situated on the north of Bay of Bengal. Climatically it is a humid subtropical country. Most of the land is deltaic plain of two great rivers, the Ganges and the Bhrammaputra and it tributaries. The country has rich diversity of plants. Main crops cultivated are Rice, Jute, Wheat, Maize, Sugarcane, Mustard and different kinds of Lentils. There are a good number of Public and Private Universities and Plant Research Establishments. Bangladesh Journal of Botany is the official organ of the Bangladesh Botanical Society established in 1972. Since 1972 Bangladesh Journal of Botany is being published regularly. Two issues of the Journal are published, one in June and another in December. Scientific papers (Full paper and short communication) on any field of Plant Sciences from anywhere in the World are considered for publication in Bangladesh Journal of Botany.