Characterization of three citrullus lanatus (thunb.) Matsum. & nakai varieties by fluorescent chromosome staining and rapd analysis

Pub Date : 2023-12-31 DOI:10.3329/bjb.v52i4.70598

C. Dash, M. Afroz, Afsana Hossain, Syeda Sharmeen Sultana, Subroto Kumar Das

引用次数: 0

Abstract

Three commercial Citrullus lanatus varieties, namely Sonya, Kanya and Sweet black-2, were investigated with cyto-molecular techniques for characterization. Despite having similar somatic chromosome numbers and karyotype formulae (2n = 22), these three varieties had considerable diversities in Chromomycin A3 (CMA)- and 4′, 6-diamidino-2-phenylindole (DAPI)-banding patterns. The total somatic chromosomal length was longest in Sweet black-2 (71.44 ± 2.94 µm) and shortest in Sonya (42.31 ± 2.45 µm). Each variety's unique GC- and AT-rich chromatin composition was represented by a variety of numbers, percentages, and patterns of distribution. Eleven random primers were employed to evaluate the genetic diversity among three varieties. Each variety showed a distinct fingerprinting pattern with 88.72% polymorphism while having 10 of the same RAPD bands. Bangladesh J. Bot. 52(4): 1033-1040, 2023 (December)

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通过荧光染色体染色和 RAPD 分析确定三个 citrullus lanatus (thunb.) Matsum.

研究人员利用细胞分子技术对三个商品瓜品种（即 Sonya、Kanya 和 Sweet black-2）进行了表征。尽管这三个品种的体细胞染色体数和核型公式（2n = 22）相似，但它们在色霉素 A3（CMA）和 4′，6-二脒基-2-苯基吲哚（DAPI）带状模式上却有很大差异。甜黑 2 号的体细胞染色体总长度最长（71.44 ± 2.94 µm），索尼娅最短（42.31 ± 2.45 µm）。每个品种都有独特的富含 GC 和 AT 的染色质组成，其数量、百分比和分布模式各不相同。采用 11 种随机引物评估了三个品种的遗传多样性。每个品种都表现出独特的指纹图谱，多态性高达 88.72%，同时有 10 条相同的 RAPD 带。孟加拉国植物学杂志52(4):1033-1040，2023 年（12 月）

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