Characterization of three citrullus lanatus (thunb.) Matsum. & nakai varieties by fluorescent chromosome staining and rapd analysis

IF 0.4 4区生物学 Q4 PLANT SCIENCES

Bangladesh Journal of Botany Pub Date : 2023-12-31 DOI:10.3329/bjb.v52i4.70598

C. Dash, M. Afroz, Afsana Hossain, Syeda Sharmeen Sultana, Subroto Kumar Das

引用次数: 0

Abstract

Three commercial Citrullus lanatus varieties, namely Sonya, Kanya and Sweet black-2, were investigated with cyto-molecular techniques for characterization. Despite having similar somatic chromosome numbers and karyotype formulae (2n = 22), these three varieties had considerable diversities in Chromomycin A3 (CMA)- and 4′, 6-diamidino-2-phenylindole (DAPI)-banding patterns. The total somatic chromosomal length was longest in Sweet black-2 (71.44 ± 2.94 µm) and shortest in Sonya (42.31 ± 2.45 µm). Each variety's unique GC- and AT-rich chromatin composition was represented by a variety of numbers, percentages, and patterns of distribution. Eleven random primers were employed to evaluate the genetic diversity among three varieties. Each variety showed a distinct fingerprinting pattern with 88.72% polymorphism while having 10 of the same RAPD bands. Bangladesh J. Bot. 52(4): 1033-1040, 2023 (December)

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通过荧光染色体染色和 RAPD 分析确定三个 citrullus lanatus (thunb.) Matsum.

研究人员利用细胞分子技术对三个商品瓜品种（即 Sonya、Kanya 和 Sweet black-2）进行了表征。尽管这三个品种的体细胞染色体数和核型公式（2n = 22）相似，但它们在色霉素 A3（CMA）和 4′，6-二脒基-2-苯基吲哚（DAPI）带状模式上却有很大差异。甜黑 2 号的体细胞染色体总长度最长（71.44 ± 2.94 µm），索尼娅最短（42.31 ± 2.45 µm）。每个品种都有独特的富含 GC 和 AT 的染色质组成，其数量、百分比和分布模式各不相同。采用 11 种随机引物评估了三个品种的遗传多样性。每个品种都表现出独特的指纹图谱，多态性高达 88.72%，同时有 10 条相同的 RAPD 带。孟加拉国植物学杂志52(4):1033-1040，2023 年（12 月）

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bangladesh Journal of Botany 生物-植物科学

CiteScore

0.80

自引率

0.00%

发文量

审稿时长

9 months

期刊介绍： Bangladesh in situated on the north of Bay of Bengal. Climatically it is a humid subtropical country. Most of the land is deltaic plain of two great rivers, the Ganges and the Bhrammaputra and it tributaries. The country has rich diversity of plants. Main crops cultivated are Rice, Jute, Wheat, Maize, Sugarcane, Mustard and different kinds of Lentils. There are a good number of Public and Private Universities and Plant Research Establishments. Bangladesh Journal of Botany is the official organ of the Bangladesh Botanical Society established in 1972. Since 1972 Bangladesh Journal of Botany is being published regularly. Two issues of the Journal are published, one in June and another in December. Scientific papers (Full paper and short communication) on any field of Plant Sciences from anywhere in the World are considered for publication in Bangladesh Journal of Botany.