Cell wall epitope distribution in the functional compartments of galls induced by Palaeomystella oligophaga (Lepidoptera) in Macairea radula (Melastomataceae)

IF 0.9 4区 生物学 Q4 PLANT SCIENCES
Patrícia Dias Santos, Gracielle Pereira Pimenta Bragança, Vinícius Coelho Kuster, Diego Ismael Rocha, Denis Coelho de Oliveira
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引用次数: 0

Abstract

Context

The parasitic interaction between the galling insect Palaeomystella oligophaga (Lepidoptera) and the host plant tissues of Macairea radula (Melastomataceae) leads to the formation of globoid galls. These galls have storage and typical nutritive tissues (outer and inner compartments, respectively), whose functions may be related to differential cell wall component distributions and to the stage of development of the gall inducer.

Aims

We evaluated whether the cell wall composition of the gall compartments (storage and nutritive tissues) changes according to the developmental stage of the gall inducer P. oligophaga.

Methods

The galls occupied by the gall inducer in different stages of development (i.e. larval and pupal stages, as well as empty galls) were collected and submitted to histological examination, immunocytochemical analyses using monoclonal antibodies for pectins, hemicelluloses, and glycoproteins, and histochemical analyses using phloroglucinol for lignins.

Key results

The histological results showed that nutritive tissues have smaller cells compared with storage ones when the galling insect is in the larval stage. Immunocytochemical analyses also showed the occurrence of both methyl-esterified and unesterified pectins, as well hemicelluloses in the nutritive tissue during the larval stage. The dynamic of some cell wall components changes when the gall inducer is in the pupal stage, or the galls are empty.

Conclusions

The changes in the composition of the cell walls, especially regarding homogalacturonans and xyloglucans, support the high metabolism and resource availability in cell walls of nutritive tissue for the gall inducer.

Implications

The cell wall composition may indicate important metabolic steps during gall formation and galling insect diet.

鳞翅目 Palaeomystella oligophaga(Palaeomystella oligophaga)诱导的马缨丹(Macairea radula)虫瘿功能区的细胞壁表位分布
背景瘿蚊 Palaeomystella oligophaga(鳞翅目)与寄主植物马缨丹(Melastomataceae)组织之间的寄生相互作用导致球形虫瘿的形成。这些虫瘿具有贮藏组织和典型的营养组织(分别为外层和内层),其功能可能与细胞壁成分的不同分布以及虫瘿诱导体的发育阶段有关。目的我们评估了虫瘿各部分(贮藏组织和营养组织)的细胞壁成分是否会随着虫瘿诱导体 P. oligophaga 的发育阶段而发生变化。方法收集虫瘿诱导体在不同发育阶段(即幼虫期和蛹期,以及空虫瘿)所占据的虫瘿,并对其进行组织学检查,使用单克隆抗体对果胶、半纤维素和糖蛋白进行免疫细胞化学分析,使用氯代葡萄糖苷对木质素进行组织化学分析。主要结果组织学结果表明,在瘿蚊幼虫阶段,营养组织的细胞比储藏组织的细胞小。免疫细胞化学分析还表明,幼虫期营养组织中存在甲基酯化和未酯化果胶以及半纤维素。当虫瘿诱导体处于蛹期或虫瘿为空时,某些细胞壁成分的动态会发生变化。结论细胞壁成分的变化,尤其是高半乳糖醛酸和木聚糖的变化,支持了虫瘿诱导体营养组织细胞壁的高代谢和资源可用性。意义细胞壁成分可能表明了虫瘿形成和虫瘿昆虫饮食过程中的重要代谢步骤。
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来源期刊
Australian Journal of Botany
Australian Journal of Botany 生物-植物科学
CiteScore
2.30
自引率
18.20%
发文量
26
审稿时长
6-12 weeks
期刊介绍: Australian Journal of Botany is an international journal for publication of original research in plant science. We seek papers of broad interest with relevance to Southern Hemisphere ecosystems. Our scope encompasses all approaches to understanding plant biology. Australian Journal of Botany is published with the endorsement of the Commonwealth Scientific and Industrial Research Organisation (CSIRO) and the Australian Academy of Science.
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