Cell wall epitope distribution in the functional compartments of galls induced by Palaeomystella oligophaga (Lepidoptera) in Macairea radula (Melastomataceae)
Patrícia Dias Santos, Gracielle Pereira Pimenta Bragança, Vinícius Coelho Kuster, Diego Ismael Rocha, Denis Coelho de Oliveira
{"title":"Cell wall epitope distribution in the functional compartments of galls induced by Palaeomystella oligophaga (Lepidoptera) in Macairea radula (Melastomataceae)","authors":"Patrícia Dias Santos, Gracielle Pereira Pimenta Bragança, Vinícius Coelho Kuster, Diego Ismael Rocha, Denis Coelho de Oliveira","doi":"10.1071/bt23064","DOIUrl":null,"url":null,"abstract":"<strong> Context</strong><p>The parasitic interaction between the galling insect <i>Palaeomystella oligophaga</i> (Lepidoptera) and the host plant tissues of <i>Macairea radula</i> (Melastomataceae) leads to the formation of globoid galls. These galls have storage and typical nutritive tissues (outer and inner compartments, respectively), whose functions may be related to differential cell wall component distributions and to the stage of development of the gall inducer.</p><strong> Aims</strong><p>We evaluated whether the cell wall composition of the gall compartments (storage and nutritive tissues) changes according to the developmental stage of the gall inducer <i>P. oligophaga</i>.</p><strong> Methods</strong><p>The galls occupied by the gall inducer in different stages of development (i.e. larval and pupal stages, as well as empty galls) were collected and submitted to histological examination, immunocytochemical analyses using monoclonal antibodies for pectins, hemicelluloses, and glycoproteins, and histochemical analyses using phloroglucinol for lignins.</p><strong> Key results</strong><p>The histological results showed that nutritive tissues have smaller cells compared with storage ones when the galling insect is in the larval stage. Immunocytochemical analyses also showed the occurrence of both methyl-esterified and unesterified pectins, as well hemicelluloses in the nutritive tissue during the larval stage. The dynamic of some cell wall components changes when the gall inducer is in the pupal stage, or the galls are empty.</p><strong> Conclusions</strong><p>The changes in the composition of the cell walls, especially regarding homogalacturonans and xyloglucans, support the high metabolism and resource availability in cell walls of nutritive tissue for the gall inducer.</p><strong> Implications</strong><p>The cell wall composition may indicate important metabolic steps during gall formation and galling insect diet.</p>","PeriodicalId":8607,"journal":{"name":"Australian Journal of Botany","volume":null,"pages":null},"PeriodicalIF":0.9000,"publicationDate":"2024-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Australian Journal of Botany","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1071/bt23064","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
Context
The parasitic interaction between the galling insect Palaeomystella oligophaga (Lepidoptera) and the host plant tissues of Macairea radula (Melastomataceae) leads to the formation of globoid galls. These galls have storage and typical nutritive tissues (outer and inner compartments, respectively), whose functions may be related to differential cell wall component distributions and to the stage of development of the gall inducer.
Aims
We evaluated whether the cell wall composition of the gall compartments (storage and nutritive tissues) changes according to the developmental stage of the gall inducer P. oligophaga.
Methods
The galls occupied by the gall inducer in different stages of development (i.e. larval and pupal stages, as well as empty galls) were collected and submitted to histological examination, immunocytochemical analyses using monoclonal antibodies for pectins, hemicelluloses, and glycoproteins, and histochemical analyses using phloroglucinol for lignins.
Key results
The histological results showed that nutritive tissues have smaller cells compared with storage ones when the galling insect is in the larval stage. Immunocytochemical analyses also showed the occurrence of both methyl-esterified and unesterified pectins, as well hemicelluloses in the nutritive tissue during the larval stage. The dynamic of some cell wall components changes when the gall inducer is in the pupal stage, or the galls are empty.
Conclusions
The changes in the composition of the cell walls, especially regarding homogalacturonans and xyloglucans, support the high metabolism and resource availability in cell walls of nutritive tissue for the gall inducer.
Implications
The cell wall composition may indicate important metabolic steps during gall formation and galling insect diet.
期刊介绍:
Australian Journal of Botany is an international journal for publication of original research in plant science. We seek papers of broad interest with relevance to Southern Hemisphere ecosystems. Our scope encompasses all approaches to understanding plant biology.
Australian Journal of Botany is published with the endorsement of the Commonwealth Scientific and Industrial Research Organisation (CSIRO) and the Australian Academy of Science.