Heterologous Production of Antimicrobial Peptides: Notes to Consider

IF 1.9 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Masoumeh Kordi, Parnian Ghaedi Talkhounche, Helia Vahedi, Naser Farrokhi, Maryam Tabarzad
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Abstract

Heavy and irresponsible use of antibiotics in the last century has put selection pressure on the microbes to evolve even faster and develop more resilient strains. In the confrontation with such sometimes called “superbugs”, the search for new sources of biochemical antibiotics seems to have reached the limit. In the last two decades, bioactive antimicrobial peptides (AMPs), which are polypeptide chains with less than 100 amino acids, have attracted the attention of many in the control of microbial pathogens, more than the other types of antibiotics. AMPs are groups of components involved in the immune response of many living organisms, and have come to light as new frontiers in fighting with microbes. AMPs are generally produced in minute amounts within organisms; therefore, to address the market, they have to be either produced on a large scale through recombinant DNA technology or to be synthesized via chemical methods. Here, heterologous expression of AMPs within bacterial, fungal, yeast, plants, and insect cells, and points that need to be considered towards their industrialization will be reviewed.

Graphical Abstract

Sources of peptide production and their applications. Some AMPs directly extracted from natural sources, some of them are chemically synthesized either using liquid or solid phase peptides synthesis, and for large scale production, recombinant expression using heterologous expression systems have been used.

抗菌肽的异源生产:注意事项。
上个世纪抗生素的大量和不负责任的使用给微生物带来了选择压力,使它们进化得更快,并发展出更有生命力的菌株。在与这些有时被称为 "超级细菌 "的微生物对抗的过程中,寻找生化抗生素新来源的努力似乎已经达到了极限。在过去的二十年里,生物活性抗菌肽(AMPs),即氨基酸少于 100 个的多肽链,在控制微生物病原体方面比其他类型的抗生素更受关注。AMPs 是参与许多生物体免疫反应的一组成分,已成为与微生物斗争的新领域。AMPs 通常在生物体内产生的量极少,因此,要满足市场需求,必须通过 DNA 重组技术进行大规模生产,或通过化学方法合成。这里将综述在细菌、真菌、酵母、植物和昆虫细胞中异源表达 AMPs 的情况,以及实现其工业化需要考虑的要点。多肽的生产来源及其应用。有些 AMP 直接从天然资源中提取,有些使用液相或固相多肽合成法进行化学合成,而大规模生产则使用异源表达系统进行重组表达。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
The Protein Journal
The Protein Journal 生物-生化与分子生物学
CiteScore
5.20
自引率
0.00%
发文量
57
审稿时长
12 months
期刊介绍: The Protein Journal (formerly the Journal of Protein Chemistry) publishes original research work on all aspects of proteins and peptides. These include studies concerned with covalent or three-dimensional structure determination (X-ray, NMR, cryoEM, EPR/ESR, optical methods, etc.), computational aspects of protein structure and function, protein folding and misfolding, assembly, genetics, evolution, proteomics, molecular biology, protein engineering, protein nanotechnology, protein purification and analysis and peptide synthesis, as well as the elucidation and interpretation of the molecular bases of biological activities of proteins and peptides. We accept original research papers, reviews, mini-reviews, hypotheses, opinion papers, and letters to the editor.
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