Preparation of [1′-13C]citric acid as a probe in a breath test to evaluate tricarboxylic acid cycle flux

IF 0.9 4区医学 Q4 BIOCHEMICAL RESEARCH METHODS

Journal of labelled compounds & radiopharmaceuticals Pub Date : 2024-01-03 DOI:10.1002/jlcr.4083

Hidemichi Mitome, Mieko Takenishi, Kumiko Ono, Naoyuki Kawagoe, Tsunehiko Imai, Yosuke Sasaki, Yoshihisa Urita, Kazuki Akira

引用次数: 0

Abstract

[1′-¹³C]Citric acid (1) was efficiently prepared from dimethyl 1,3-acetonedicarboxylate in two steps as a probe for a breath test. The synthetic method was selected because of the yield and reproducibility. Compound 1 was orally administrated to rats, and the time course of the increase of ¹³CO₂/¹²CO₂ ratios (Δ¹³CO₂) in their breath was successfully followed, indicating the metabolism of 1. Thus, the ¹³C-breath test using 1 is a promising method to evaluate tricarboxylic acid (TCA) cycle flux.

Abstract Image

查看原文本刊更多论文

制备[1'-13 C]柠檬酸作为呼气试验的探针，以评估三羧酸循环通量。

[1'-13 C]柠檬酸（1）由 1,3-丙酮二羧酸二甲酯通过两个步骤高效制备而成，可作为呼气测试的探针。之所以选择这种合成方法，是因为其产量高且具有可重复性。给大鼠口服化合物 1，成功地跟踪了大鼠呼气中 13 CO2 /12 CO2 比率（Δ13 CO2）增加的时间过程，表明了 1 的新陈代谢。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of labelled compounds & radiopharmaceuticals 医学-分析化学

CiteScore

3.30

自引率

0.00%

发文量

审稿时长

1 months

期刊介绍： The Journal of Labelled Compounds and Radiopharmaceuticals publishes all aspects of research dealing with labeled compound preparation and applications of these compounds. This includes tracer methods used in medical, pharmacological, biological, biochemical and chemical research in vitro and in vivo. The Journal of Labelled Compounds and Radiopharmaceuticals devotes particular attention to biomedical research, diagnostic and therapeutic applications of radiopharmaceuticals, covering all stages of development from basic metabolic research and technological development to preclinical and clinical studies based on physically and chemically well characterized molecular structures, coordination compounds and nano-particles.