Repairing Effect of Mesenchymal Stem Cells on Lead Acetate-Induced Testicular Injury in Mice.

IF 3.2 4区医学 Q3 CELL & TISSUE ENGINEERING

Cell Transplantation Pub Date : 2024-01-01 DOI:10.1177/09636897231219395

Shasha Zhao, Zhaozhi Li, Kun Li, Xiaoyu Dai, Zhe Xu, Li Li, Huanhuan Wang, Xiaodun Liu, Dong Li

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引用次数: 0

Abstract

Lead acetate can cause testicular damage in males. In this study, we assessed the repairing effects of human umbilical cord mesenchymal stem cells (MSCs) on testicular injury caused by lead acetate in mice. MSCs were injected into mice with testicular injury by intraperitoneal injection, and the organ coefficient of reproductive organs, sperm motility, hormone level and antioxidant index of mice were tested. Compared with the normal group, the coefficient of reproductive organs and sperm motility were reduced in the model group, and histopathology showed obvious testicular injury, proving successful modeling. Compared with the model group, the reproductive organ coefficient and sperm motility were improved in the experimental group, and histopathology showed that the testicular injury could be significantly improved. Sex hormone secretion tends to be normal, and the antioxidant index increased. Sequencing results showed that there were 485 upregulated genes and 172 downregulated genes between the model group and the control group, and 210 upregulated genes and 482 downregulated genes between the experimental group and the model group. Differentially expressed genes are mainly concentrated in AMP-activated protein kinase (AMPK) signaling pathway, apoptosis signaling pathway, and arginine biosynthesis signaling pathway. Overall, MSCs can significantly improve the degree of damages to mice testis caused by lead acetate and have a certain repairing effect.

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间充质干细胞对醋酸铅诱发的小鼠睾丸损伤的修复作用

醋酸铅可导致男性睾丸损伤。本研究评估了人脐带间充质干细胞（MSCs）对醋酸铅引起的小鼠睾丸损伤的修复作用。将间充质干细胞腹腔注射到睾丸损伤的小鼠体内，检测小鼠生殖器官系数、精子活力、激素水平和抗氧化指数。与正常组相比，模型组的生殖器官系数和精子活力降低，组织病理学显示睾丸损伤明显，证明造模成功。与模型组相比，实验组的生殖器官系数和精子活力均有所提高，组织病理学显示睾丸损伤得到明显改善。性激素分泌趋于正常，抗氧化指数上升。测序结果显示，模型组和对照组之间有 485 个上调基因和 172 个下调基因，实验组和模型组之间有 210 个上调基因和 482 个下调基因。差异表达基因主要集中在AMPK信号通路、细胞凋亡信号通路和精氨酸生物合成信号通路。总之，间充质干细胞能明显改善醋酸铅对小鼠睾丸的损伤程度，并具有一定的修复作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cell Transplantation 生物-细胞与组织工程

CiteScore

6.00

自引率

3.00%

发文量

审稿时长

6 months

期刊介绍： Cell Transplantation, The Regenerative Medicine Journal is an open access, peer reviewed journal that is published 12 times annually. Cell Transplantation is a multi-disciplinary forum for publication of articles on cell transplantation and its applications to human diseases. Articles focus on a myriad of topics including the physiological, medical, pre-clinical, tissue engineering, stem cell, and device-oriented aspects of the nervous, endocrine, cardiovascular, and endothelial systems, as well as genetically engineered cells. Cell Transplantation also reports on relevant technological advances, clinical studies, and regulatory considerations related to the implantation of cells into the body in order to provide complete coverage of the field.