Prevalence estimation of Tropheryma whipplei in duodenal biopsy tissues of Koreans.

IF 4.6 2区 医学 Q1 MICROBIOLOGY
Sumi Yoon, Min Eui Hong, Soon Auck Hong, Tae-Hyoung Kim, Mi-Kyung Lee
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引用次数: 0

Abstract

Whipple's disease caused by Tropheryma whipplei is difficult to diagnose because of a broad spectrum of manifestations and non-specific clinical signs. In the current global era, the incidence of duodenal infection/inflammation caused by T. whipplei in Korea may has been underestimated. Here we estimated the prevalence of T. whipplei in duodenal biopsy tissues of Koreans using real-time PCRs (RT-PCRs). A total of 252 duodenal biopsy tissues were collected from Korean patients who underwent esophagogastroduodenoscopy and duodenal biopsy. DNA extracted from the duodenal biopsy tissues was analyzed using three RT-PCRs targeting T. whipplei-specific regions of the 16S-23S rRNA intergenic spacer, hsp65, and Dig15 in parallel. In the samples positive in RT-PCRs, direct sequencing was performed for each RT-PCR target. The prevalence of T. whipplei was estimated based on the RT-PCR and sequencing results. Among the analyzed samples, T. whipplei was not detected. The prevalence of T. whipplei in duodenal biopsy tissues of Koreans was estimated to be less than 0.4%. This is the first study to attempt to detect T. whipplei in duodenal biopsy tissues of Koreans and estimate its prevalence. Our findings infer that while T. whipplei carriers exist in Korea, the incidence of duodenal infection/inflammation caused by T. whipplei is extremely rare.

对韩国人十二指肠活检组织中白痢疾杆菌流行率的估计。
由惠氏梭菌(Tropheryma whipplei)引起的惠普尔病很难诊断,因为该病的表现范围很广,而且临床症状不具特异性。在当今全球时代,韩国由惠氏梭菌引起的十二指肠感染/炎症的发病率可能被低估了。在此,我们使用实时 PCR(RT-PCR)技术估算了韩国人十二指肠活检组织中的白喉杆菌感染率。我们从接受食管胃十二指肠镜检查和十二指肠活检的韩国患者身上共收集了 252 份十二指肠活检组织。从十二指肠活检组织中提取的 DNA 采用三种 RT-PCR 方法进行分析,这些 RT-PCR 方法分别针对 16S-23S rRNA 基因间距、hsp65 和 Dig15 中的 T. whipplei 特异性区域。在 RT-PCR 检测结果呈阳性的样本中,对每个 RT-PCR 靶区都进行了直接测序。根据 RT-PCR 和测序结果估算了白喉杆菌的流行率。在分析的样本中,没有检测到白喉杆菌。据估计,韩国人十二指肠活检组织中的白喉杆菌感染率低于 0.4%。这是首次尝试检测韩国人十二指肠活检组织中的白喉杆菌并估计其流行率的研究。我们的研究结果推断,虽然韩国存在白喉杆菌携带者,但由白喉杆菌引起的十二指肠感染/炎症的发病率极为罕见。
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来源期刊
CiteScore
8.60
自引率
0.00%
发文量
49
审稿时长
>12 weeks
期刊介绍: Annals of Clinical Microbiology and Antimicrobials considers good quality, novel and international research of more than regional relevance. Research must include epidemiological and/or clinical information about isolates, and the journal covers the clinical microbiology of bacteria, viruses and fungi, as well as antimicrobial treatment of infectious diseases. Annals of Clinical Microbiology and Antimicrobials is an open access, peer-reviewed journal focusing on information concerning clinical microbiology, infectious diseases and antimicrobials. The management of infectious disease is dependent on correct diagnosis and appropriate antimicrobial treatment, and with this in mind, the journal aims to improve the communication between laboratory and clinical science in the field of clinical microbiology and antimicrobial treatment. Furthermore, the journal has no restrictions on space or access; this ensures that the journal can reach the widest possible audience.
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