Prognostic Role of Mitochondrial Transcription Termination Factor 3 in Thyroid Carcinoma.

IF 1.1 4区 生物学 Q4 GENETICS & HEREDITY
Mei-Tao Sun, Heng-Yu Zhao, Hua-Juan Ruan, Li-Hui Yu, Ming-Li Guan, Jun-Jie Fan, Chen-Zhuo Feng, Yang-Yun Lou
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引用次数: 0

Abstract

Background: Studies have shown that the Mitochondrial Transcription Termination Factor 3 (MTERF3) negatively regulates mitochondrial gene expression and energy metabolism, and plays a significant role in many cancer types. Nevertheless, the expression and prognostic role of MTERF3 in patients with thyroid carcinoma (THCA) is still unclear. Thus, we investigated the expression, clinicopathological significance, and prognostic value of MTERF3 in THCA. Methods: The protein and mRNA expression levels of MTERF3 were, respectively, analyzed using immunohistochemistry (IHC) from THCA tissues and RNA-Seq data downloaded from The Cancer Genome Atlas. In addition, the relationships among the expression of MTERF3, the stemness feature, the extent of immune infiltration, drug sensitivity, the expression of ferroptosis, and N6-methyladenosine (m6A) methylation regulators, were evaluated as prognostic indicators for patients with THCA using the Kaplan-Meier plotter database. Results: The IHC and RNAseq results showed that the protein and mRNA expression levels of MTERF3 in adjacent nontumor tissues were significantly higher than in THCA tissues. The survival analysis indicated that decreased expression of MTERF3 was associated with a poorer prognosis. Furthermore, the expression of MTERF3 not only negatively correlated with the enhancement of the stemness of THCA and the reduction of drug sensitivity but also was implicated in ferroptosis and m6A methylation. Conclusion: The data from this study support the hypothesis that decreased expression of MTERF3 in THCA is associated with a poor prognosis.

线粒体转录终止因子 3 在甲状腺癌中的预后作用
背景:研究表明,线粒体转录终止因子3(MTERF3)负性调控线粒体基因表达和能量代谢,并在多种癌症类型中发挥重要作用。然而,MTERF3在甲状腺癌(THCA)患者中的表达和预后作用仍不清楚。因此,我们研究了MTERF3在甲状腺癌中的表达、临床病理意义和预后价值。研究方法利用免疫组化(IHC)技术和从癌症基因组图谱(The Cancer Genome Atlas)下载的RNA-Seq数据,分别分析了THCA组织中MTERF3的蛋白和mRNA表达水平。此外,还利用 Kaplan-Meier plotter 数据库评估了 MTERF3 的表达、干性特征、免疫浸润程度、药物敏感性、铁突变表达和 N6-甲基腺苷(m6A)甲基化调节因子之间的关系,并将其作为 THCA 患者的预后指标。结果IHC和RNAseq结果显示,邻近非肿瘤组织中MTERF3的蛋白和mRNA表达水平明显高于THCA组织。生存分析表明,MTERF3表达量减少与预后较差有关。此外,MTERF3 的表达不仅与 THCA 干性的增强和药物敏感性的降低呈负相关,还与铁变态反应和 m6A 甲基化有关。结论本研究的数据支持了 THCA 中 MTERF3 表达减少与预后不良有关的假设。
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来源期刊
CiteScore
2.50
自引率
7.10%
发文量
63
审稿时长
1 months
期刊介绍: Genetic Testing and Molecular Biomarkers is the leading peer-reviewed journal covering all aspects of human genetic testing including molecular biomarkers. The Journal provides a forum for the development of new technology; the application of testing to decision making in an increasingly varied set of clinical situations; ethical, legal, social, and economic aspects of genetic testing; and issues concerning effective genetic counseling. This is the definitive resource for researchers, clinicians, and scientists who develop, perform, and interpret genetic tests and their results. Genetic Testing and Molecular Biomarkers coverage includes: -Diagnosis across the life span- Risk assessment- Carrier detection in individuals, couples, and populations- Novel methods and new instrumentation for genetic testing- Results of molecular, biochemical, and cytogenetic testing- Genetic counseling
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