Time-reversal symmetry breaking in the chemosensory array: asymmetric switching and dissipation-enhanced sensing

Abstract

The Escherichia coli chemoreceptors form an extensive array that achieves cooperative and adaptive sensing of extracellular signals. The receptors control the activity of histidine kinase CheA, which drives a non-equilibrium phosphorylation-dephosphorylation reaction cycle for response regulator CheY. Recent single-cell FRET measurements revealed that kinase activity of the array spontaneously switches between active and inactive states, with asymmetric switching times that signify time-reversal symmetry breaking in the underlying dynamics. Here, we show that the asymmetric switching dynamics can be explained by a non-equilibrium lattice model, which considers both the dissipative reaction cycles of individual core units and the coupling between neighboring units. The model reveals that large dissipation and near-critical coupling are required to explain the observed switching dynamics. Microscopically, the switching time asymmetry originates from irreversible transition paths. The model shows that strong dissipation enables sensitive and rapid signaling response by relieving the speed-sensitivity trade-off, which can be tested by future single-cell experiments. Overall, our model provides a general framework for studying biological complexes composed of coupled subunits that are individually driven by dissipative cycles and the rich non-equilibrium physics within.