Apigenin Release from Chitosan/Gelatin Membranes Promotes Osteogenic Differentiation of Adipose-Derived Mesenchymal Stem Cells

IF 1.6 4区医学 Q4 ENGINEERING, BIOMEDICAL

Journal of Medical and Biological Engineering Pub Date : 2023-12-25 DOI:10.1007/s40846-023-00832-w

Azam Bozorgi, Mozafar Khazaei, Maryam Bozorgi, Zahra Jamalpoor

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引用次数: 0

Abstract

Purpose

Apigenin is a widespread phytochemical with beneficial effects on osteoblastic differentiation. However, short half-life and unstable chemical structure restrict apigenin application in bone tissue engineering applications. Here, we investigated the impact of apigenin-loaded chitosan/gelatin (Api. Cs/Gel) membranes on the osteogenic differentiation of human adipose-derived mesenchymal stem cells (hADMSCs).

Methods

Api10. Cs/Gel and Api25. Cs/Gel membranes were fabricated using the solution casting method, followed by characterizing their physicochemical and biological properties. hADMSCs were isolated from healthy donors and characterized using flow cytometry. hADMSCs were seeded onto Api. Cs/Gel membranes and cultured under osteogenic differentiation for 7 and 21 days. The expression of osteogenic markers ALP, RUNX2, OCN, and COL1 was assessed using real-time PCR, and calcium mineralization was analyzed using the quantitative Alizarin red S staining.

Results

The Api. Cs/Gel membranes were successfully fabricated, and characterization data confirmed their structural uniformity, chemical homogeneity, cross-linking, and apigenin incorporation. Membranes exhibited favorable degradation, swelling ratio, and long-term apigenin release. The membranes were non-toxic and supported hADMSCs attachment, viability, and proliferation. The expression of ALP, RUNX2, OCN, and COL1 and cellular mineralization increased on day 21, and significant overexpression was observed in hADMSCs seeded onto Apigenin-loaded membranes.

Conclusion

Cs/Gel membranes provided an approving matrix for cellular interactions and apigenin inclusion, followed by a sustained release associated with enhanced osteogenic differentiation of hADMSCs and matrix mineralization.

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壳聚糖/明胶膜释放的芹菜素能促进脂肪来源间充质干细胞的成骨分化

目的芹菜素是一种广泛使用的植物化学物质，对成骨细胞分化具有有益作用。然而，芹菜素的半衰期短和化学结构不稳定限制了它在骨组织工程中的应用。在此，我们研究了芹菜素负载壳聚糖/明胶（Api. Cs/Gel）膜对人脂肪间充质干细胞（hADMSCs）成骨分化的影响。Cs/Gel和Api25.从健康供体中分离出 hADMSCs，并使用流式细胞术对其进行鉴定。Cs/Gel 膜上，并在成骨分化条件下培养 7 天和 21 天。使用实时 PCR 评估成骨标志物 ALP、RUNX2、OCN 和 COL1 的表达，使用定量茜素红 S 染色分析钙矿化。Cs/Gel膜成功制成，表征数据证实了它们的结构均匀性、化学均匀性、交联性和芹菜素掺入性。膜表现出良好的降解性、溶胀率和长期芹菜素释放。膜无毒，支持 hADMSCs 的附着、存活和增殖。ALP、RUNX2、OCN 和 COL1 的表达以及细胞矿化在第 21 天有所增加，在芹菜素负载膜上播种的 hADMSCs 中观察到显著的过表达。

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来源期刊

Journal of Medical and Biological Engineering ENGINEERING, BIOMEDICAL-

CiteScore

4.30

自引率

5.00%

发文量

审稿时长

3 months

期刊介绍： The purpose of Journal of Medical and Biological Engineering, JMBE, is committed to encouraging and providing the standard of biomedical engineering. The journal is devoted to publishing papers related to clinical engineering, biomedical signals, medical imaging, bio-informatics, tissue engineering, and so on. Other than the above articles, any contributions regarding hot issues and technological developments that help reach the purpose are also included.