Fangke Xu, Jason Y Tann, Oliver R Wilkes, Adrian W Moore
{"title":"Filleting and Immunostaining of Larvae to Visualize <i>Drosophila</i> Dendritic Arborization Neuron Dendrite Arbors.","authors":"Fangke Xu, Jason Y Tann, Oliver R Wilkes, Adrian W Moore","doi":"10.1101/pdb.prot108148","DOIUrl":null,"url":null,"abstract":"<p><p>Nervous system formation involves the specification of neuron identity, followed by precise circuit construction; this includes controlling the pattern and connectivity of the dendrite arbor. <i>Drosophila</i> dendritic arborization (da) neurons are a powerful experimental model for studying dendrite arbor differentiation mechanisms. da neuron dendrite arbors elaborate in two dimensions in the body wall, making it easy to visualize them with high resolution. Immunostaining is a conventional method to examine arbor pattern and the subcellular distribution of proteins. In addition, images acquired from immunostaining protocols can amplify weaker signals from fluorescent transgenic proteins and be used to quantify protein expression levels. This protocol describes a broadly applicable dissection, fixation, and immunostaining approach in <i>Drosophila</i> larvae.</p>","PeriodicalId":10496,"journal":{"name":"Cold Spring Harbor protocols","volume":" ","pages":"pdb.prot108148"},"PeriodicalIF":0.0000,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cold Spring Harbor protocols","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1101/pdb.prot108148","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Nervous system formation involves the specification of neuron identity, followed by precise circuit construction; this includes controlling the pattern and connectivity of the dendrite arbor. Drosophila dendritic arborization (da) neurons are a powerful experimental model for studying dendrite arbor differentiation mechanisms. da neuron dendrite arbors elaborate in two dimensions in the body wall, making it easy to visualize them with high resolution. Immunostaining is a conventional method to examine arbor pattern and the subcellular distribution of proteins. In addition, images acquired from immunostaining protocols can amplify weaker signals from fluorescent transgenic proteins and be used to quantify protein expression levels. This protocol describes a broadly applicable dissection, fixation, and immunostaining approach in Drosophila larvae.
Cold Spring Harbor protocolsBiochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
3.00
自引率
0.00%
发文量
163
期刊介绍：
Cold Spring Harbor Laboratory is renowned for its teaching of biomedical research techniques. For decades, participants in its celebrated, hands-on courses and users of its laboratory manuals have gained access to the most authoritative and reliable methods in molecular and cellular biology. Now that access has moved online. Cold Spring Harbor Protocols is an interdisciplinary journal providing a definitive source of research methods in cell, developmental and molecular biology, genetics, bioinformatics, protein science, computational biology, immunology, neuroscience and imaging. Each monthly issue details multiple essential methods—a mix of cutting-edge and well-established techniques.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
