Microbiome analysis of bile samples in patients with choledocholithiasis and hepatobiliary disorders.

IF 1.7 Q3 INFECTIOUS DISEASES
GERMS Pub Date : 2023-09-30 eCollection Date: 2023-09-01 DOI:10.18683/germs.2023.1390
Masoumeh Azimirad, Amir Sadeghi, Nazanin Hosseinkhan, Seyedeh Zohre Mirbagheri, Masoud Alebouyeh
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引用次数: 0

Abstract

Introduction: The involvement of bacteria in the pathogenesis of biliary tract disease is largely unknown. In this study, we investigated the microbiota of the biliary tissue among adult patients with choledocholithiasis during endoscopic retrograde cholangiography (ERCP).

Methods: 16S rDNA sequencing of bile samples, culture, and data of the medication history, underlying diseases, and liver function tests were used for the interpretation of differences in the composition of detected bacterial taxa.

Results: The four most common phyla in the bile samples included Proteobacteria, Firmicutes, Actinobacteria, and Bacteroidetes. Infection with anaerobic and microaerophilic bacteria showed host specificity, where Fusobacterium, Prevotella, Veillonella, Propionibacterium, Gemella, and Helicobacter coexist in the same patients. Clostridium and Peptoclostridium spp. were detected in 80% and 86% of the patients, where the highest relative abundance rates were detected in patients with elevated alkaline phosphatase (ALP) levels and leukocytosis, respectively. Higher diversity in the bacterial population was detected in patients with common bile duct (CBD) stone, in which the richness of an unclassified member of Alphaproteobacteria plus Helicobacter, Enterobacter/Cronobacter spp., Sphingomonas, Prevotella, Fusobacterium and Aeromonas were detected.

Conclusions: Our findings suggested correlations between the presence and relative abundance of several bacterial taxa and CBD stone formation and the effect of medication and underlying diseases on the bile microbial communities. A study on a higher number of bile samples from patients compared with the control group could reveal the role of these bacteria in the pathogenesis of biliary tract disease.

胆总管结石和肝胆疾病患者胆汁样本的微生物组分析。
导言:细菌参与胆道疾病的发病机制在很大程度上尚属未知。本研究调查了内镜逆行胆管造影术(ERCP)中胆道组织中的微生物群。方法:对胆汁样本进行 16S rDNA 测序、培养,并利用用药史、基础疾病和肝功能检查数据解释检测到的细菌类群组成的差异:结果:胆汁样本中最常见的四个菌门包括变形菌门、固形菌门、放线菌门和类杆菌门。厌氧菌和微嗜氧菌感染显示出宿主特异性,在同一患者中同时存在镰刀菌、普雷沃特菌、维龙菌、丙酸杆菌、双子叶菌和螺旋杆菌。在 80% 和 86% 的患者中检测到了梭状芽孢杆菌属和肽梭状芽孢杆菌属,在碱性磷酸酶(ALP)水平升高和白细胞增多的患者中检测到的相对丰度最高。在总胆管(CBD)结石患者中检测到的细菌群多样性较高,其中有一种未分类的阿尔法蛋白杆菌,另外还有螺旋杆菌、肠杆菌属/产气杆菌属、斯芬格单胞菌属、普雷沃特氏菌属、镰刀菌属和气单胞菌属:我们的研究结果表明,一些细菌类群的存在和相对丰度与 CBD 结石的形成以及药物和潜在疾病对胆汁微生物群落的影响之间存在相关性。与对照组相比,对更多患者的胆汁样本进行研究可揭示这些细菌在胆道疾病发病机制中的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
GERMS
GERMS INFECTIOUS DISEASES-
CiteScore
2.80
自引率
5.00%
发文量
36
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