Metabolic and transcriptomic effects of Aphanothece sp. biostimulant on tomato plant growth and phosphorus acquisition

IF 2.6 3区农林科学 Q1 AGRONOMY

Journal of Plant Nutrition and Soil Science Pub Date : 2023-12-22 DOI:10.1002/jpln.202300369

Chanda Mutale-joan, Farid Rachidi, Najib El Mernissi, Abderrahim Aasfar, Hicham El Hadi, Laila Sbabou, Karim Lyamlouli, Hicham El Arroussi

{"title":"Metabolic and transcriptomic effects of Aphanothece sp. biostimulant on tomato plant growth and phosphorus acquisition","authors":"Chanda Mutale-joan, Farid Rachidi, Najib El Mernissi, Abderrahim Aasfar, Hicham El Hadi, Laila Sbabou, Karim Lyamlouli, Hicham El Arroussi","doi":"10.1002/jpln.202300369","DOIUrl":null,"url":null,"abstract":"<div>\n \n \n <section>\n \n <h3> Background</h3>\n \n <p>Phosphorus (P) fertilizers, made from rock phosphate, increase crop yields. However, rock phosphate is a finite resource, stressing the need for more P-efficient crops.</p>\n </section>\n \n <section>\n \n <h3> Aims</h3>\n \n <p>This study aims to exploit P-adaptive traits that enhance P-acquisition and P-use efficiency in crops, as a potential sustainable P-use management method in agricultural settings.</p>\n </section>\n \n <section>\n \n <h3> Methods</h3>\n \n <p>This study investigates the biostimulant effects of <i>Aphanothece</i> sp. extracts (ApE) on P absorption efficiency traits of tomato plants supplied with triple super phosphate fertilizer (0.3, 0.6, and 1.2 mM Pi).</p>\n </section>\n \n <section>\n \n <h3> Results</h3>\n \n <p>ApE extracts exhibited significant effects on P uptake, <i>Lycopersicum esculentum</i> high-affinity phosphate transporter (LePT) genes, chlorophyll, and lipid contents, compared with control plants. ApE significantly enhanced leaf LePT4 in ApE-treated plants supplied with 0.3 mM Pi, and root <i>LePT2</i> in ApE-treated plants supplied with 0.6 mM Pi. According to principal component analysis, P concentration in roots (root Pi) was closely associated with root dry weight (DW), root <i>LePT2</i>, and leaf <i>LePT4</i>. The phytohormone, gibberellin gibberellic acid 4, slightly correlated with root Pi, <i>LePT2</i> (0.18), <i>LePT4</i> (0.28), and more with root (0.72) and shoot DW (0.60), whereas abscisic acid correlated with chlorophyll content (0.60), <i>LePT2</i> (0.50), and <i>LePT4</i> (0.49), and slightly with root Pi (0.22). Gas chromatography–mass spectrometry analysis also showed that the application of ApE to plants stimulated the biosynthesis of several metabolites. The metabolic pathway analysis using MetaboAnalyst revealed that ApE treatment induced the unsaturated fatty acid, steroid, cutin, suberin, and wax biosynthesis pathways.</p>\n </section>\n \n <section>\n \n <h3> Conclusions</h3>\n \n <p>ApE application to tomato plants stimulated P uptake by roots through enhanced expression of high-affinity P transporters (root <i>LePT2</i> and leaf <i>LePT4</i>), positively correlated with root DW and root Pi. The study also suggests that algal extracts stimulate plant growth through improved P uptake and modulate the plant's metabolic pathways that favor crop performance in tomato plants under normal Pi conditions.</p>\n </section>\n </div>","PeriodicalId":16802,"journal":{"name":"Journal of Plant Nutrition and Soil Science","volume":"187 2","pages":"233-246"},"PeriodicalIF":2.6000,"publicationDate":"2023-12-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Plant Nutrition and Soil Science","FirstCategoryId":"97","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/jpln.202300369","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRONOMY","Score":null,"Total":0}

引用次数: 0

Abstract

Background

Phosphorus (P) fertilizers, made from rock phosphate, increase crop yields. However, rock phosphate is a finite resource, stressing the need for more P-efficient crops.

Aims

This study aims to exploit P-adaptive traits that enhance P-acquisition and P-use efficiency in crops, as a potential sustainable P-use management method in agricultural settings.

Methods

This study investigates the biostimulant effects of Aphanothece sp. extracts (ApE) on P absorption efficiency traits of tomato plants supplied with triple super phosphate fertilizer (0.3, 0.6, and 1.2 mM Pi).

Results

ApE extracts exhibited significant effects on P uptake, Lycopersicum esculentum high-affinity phosphate transporter (LePT) genes, chlorophyll, and lipid contents, compared with control plants. ApE significantly enhanced leaf LePT4 in ApE-treated plants supplied with 0.3 mM Pi, and root LePT2 in ApE-treated plants supplied with 0.6 mM Pi. According to principal component analysis, P concentration in roots (root Pi) was closely associated with root dry weight (DW), root LePT2, and leaf LePT4. The phytohormone, gibberellin gibberellic acid 4, slightly correlated with root Pi, LePT2 (0.18), LePT4 (0.28), and more with root (0.72) and shoot DW (0.60), whereas abscisic acid correlated with chlorophyll content (0.60), LePT2 (0.50), and LePT4 (0.49), and slightly with root Pi (0.22). Gas chromatography–mass spectrometry analysis also showed that the application of ApE to plants stimulated the biosynthesis of several metabolites. The metabolic pathway analysis using MetaboAnalyst revealed that ApE treatment induced the unsaturated fatty acid, steroid, cutin, suberin, and wax biosynthesis pathways.

Conclusions

ApE application to tomato plants stimulated P uptake by roots through enhanced expression of high-affinity P transporters (root LePT2 and leaf LePT4), positively correlated with root DW and root Pi. The study also suggests that algal extracts stimulate plant growth through improved P uptake and modulate the plant's metabolic pathways that favor crop performance in tomato plants under normal Pi conditions.

Abstract Image

查看原文本刊更多论文

Aphanothece sp.生物刺激剂对番茄植物生长和磷获取的代谢和转录组学效应

由磷矿石制成的磷（P）肥料可以提高作物产量。然而，磷矿石是一种有限的资源，因此需要更多的节磷作物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Plant Nutrition and Soil Science 农林科学-农艺学

CiteScore

4.70

自引率

8.00%

发文量

审稿时长

8-16 weeks

期刊介绍： Established in 1922, the Journal of Plant Nutrition and Soil Science (JPNSS) is an international peer-reviewed journal devoted to cover the entire spectrum of plant nutrition and soil science from different scale units, e.g. agroecosystem to natural systems. With its wide scope and focus on soil-plant interactions, JPNSS is one of the leading journals on this topic. Articles in JPNSS include reviews, high-standard original papers, and short communications and represent challenging research of international significance. The Journal of Plant Nutrition and Soil Science is one of the world’s oldest journals. You can trust in a peer-reviewed journal that has been established in the plant and soil science community for almost 100 years. Journal of Plant Nutrition and Soil Science (ISSN 1436-8730) is published in six volumes per year, by the German Societies of Plant Nutrition (DGP) and Soil Science (DBG). Furthermore, the Journal of Plant Nutrition and Soil Science (JPNSS) is a Cooperating Journal of the International Union of Soil Science (IUSS). The journal is produced by Wiley-VCH. Topical Divisions of the Journal of Plant Nutrition and Soil Science that are receiving increasing attention are: JPNSS – Topical Divisions Special timely focus in interdisciplinarity: - sustainability & critical zone science. Soil-Plant Interactions: - rhizosphere science & soil ecology - pollutant cycling & plant-soil protection - land use & climate change. Soil Science: - soil chemistry & soil physics - soil biology & biogeochemistry - soil genesis & mineralogy. Plant Nutrition: - plant nutritional physiology - nutrient dynamics & soil fertility - ecophysiological aspects of plant nutrition.