STUDY OF MICROSATELLITE LOCI POLYMORPHISM OF GRAPE SAMPLES WITH UNIDENTIFIED VARIETAL AFFILATION OF MAGARACH AMPELOGRAPHIC COLLECTION USING THE MOLECULAR MARKERS

Abstract

The paper presents the results of DNA profiling of 28 grape samples with unidentified varietal affiliation, selected from the Magarach Ampelographic Collection, and provides polymorphism assessment of their microsatellite loci. Genotyping was performed using 9 nuclear (VVMD5, VVMD7, VVMD25, VVMD27, VVMD28, VVMD32, VrZAG62, VrZAG79, VVS2) and 3 chloroplast (ccmp3, ccmp5, ccmp10) SSR markers. For nuclear loci, 105 alleles were identified, the average number of alleles per locus (na) was 11.7; the average effective number of alleles (ne) is 7.23; Shannon information index (I) for the studied batch of samples was 2.13. The highest allele frequency was observed in samples with VVS2133 and VVS2135 (p = 0.250), VVMD5238 (p = 0.232), VVMD7247 (p = 0.232), VVMD25239 (p = 0.250), VVMD27180 (p = 0.232), VVMD28236 (p = 0.143), VVMD32272 (p = 0.304), VrZAG62188 (p = 0.179), VrZAG79251 (p = 0.232) alleles. The average value of actual heterozygosity Heto was 0.8333, expected heterozygosity Hetе was 0.8712. For chloroplast loci, 7 alleles were identified: ccmp3106, ccmp3107, ccmp5104, ccmp5105, ccmp10114, ccmp10115, ccmp10116. Also 4 chlorotypes were identified: A (1 sample), B (9), C (13), D (5). A dendrogram of genetic similarity was constructed using the UPGMA method, which showed the presence of three clusters containing, respectively, 11, 11 and 6 samples. There was a uniform distribution of chlorotypes B and D over three clusters, and chlorotype C – over two clusters. It is concluded that the results can be used to assess the genetic diversity of Magarach Ampelographic Collection.